PENGEMBANGAN DAN VALIDASI METODE ANALISIS PENENTUAN KADAR ASAM FERULAT DALAM SERUM WAJAH
Ferulic acid or 4-hydroxy-3-methoxycinnamic acid is one of the most common phenolic compounds in plants. It has various activity including antimicrobial, antiarrhythmic, anti-inflammatory, antithrombotic, anticancer, antidiabetic, and antioxidant. As an antioxidant, ferulic acid is widely used in...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/66151 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Ferulic acid or 4-hydroxy-3-methoxycinnamic acid is one of the most common phenolic compounds in
plants. It has various activity including antimicrobial, antiarrhythmic, anti-inflammatory, antithrombotic, anticancer, antidiabetic, and antioxidant. As an antioxidant, ferulic acid is widely used in
the cosmetic industry for skin care products with anti-aging claims. The increasing use of anti-aging has
led to the development of anti-aging products, including anti-aging products made from ferulic acid.
Therefore, a method that can determine the content of ferulic acid in the product was needed so that
the quality, safety, and efficacy of the product can be maintained. This research was conducted to
develop a method for determining ferulic acid content in serum products using High Performance Liquid
Chromatography (HPLC). The research was started by optimizing mobile phase ratio, system suitability
test, and method validation including specificity, linearity, intraday and interday precision, and
accuracy. Based on the optimization results, acetonitrile: 0,1% (v/v) phosphoric acid (40:60) was used
as mobile phase, with isocratic method, Fortis C18 column as the stationary phase, injection volume of
10 µl, flow rate of 1 mL/min, and wavelength of 319 nm. Linearity parameter is showed by correlation
coefficient value of 0,9992 with a coefficient of variance of 2.382%. The detection and quantification
limits of ferulic acid were also obtained at 0,009 µg/mL and 0,027 µg/mL. For intraday and interday
precision, the relative standard deviation values were 0,163%-0,174%, and 0,153%-0,398%. The
recovery for accuracy was in the range of 102,423 –108,185%. The method was then used to determine
the levels of ferulic acid in the samples and the results were 0,143%, 0,172%, and 1,609%, respectively.
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