PRELIMINARY STUDY OF ASIATICOSIDE IMPRINTED POLYMER SYNTHESIS AND CHARACTERIZATION BASED ON 4-VINYLPYRIDINE AND ETHYLENE GLYCOL DIMETHACRYLATE

• Main Author: Jacinda Gya Aulia, Helga
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/68192
• Institution: Institut Teknologi Bandung

Centella asiatica has been widely used as an ingredient in skin care products because it contains several bioactive compounds. Asiaticoside is one of the bioactive compounds of the four main triterpenoids in Centella asiatica. The bioactivity of this compound is useful for wound healing and skin care. The many uses of asiaticoside for beauty and health products must be accompanied by an effective and efficient separation method. The molecularly imprinted polymer (MIP) method used in this study can serve as a preliminary study of the development of the MIP method for asiaticoside separation. MIP is a polymer that has a special cavity as a binding site designed with the molecule template. In this study, asiaticoside was used as a template molecule. MIP synthesis requires the presence of functional monomers to form bonds with template molecules and crosslinkers to form polymer chains. The functional monomer that can be used is 4-vinylpyridine (4-VP) with ethylene glycol dimethacrylate (EGDMA) crosslinker. Non-imprinted polymers (NIP) were synthesized as a comparison for MIP. The results of the synthesis were characterized using fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The MIP that was successfully formed was washed using dimethyl sulfonate (DMSO) and methanol-water (1:1, v/v) to dissolve the mold molecules. MIPL-D, MIPL-M, and NIP performance were tested on samples containing asiaticoside. The performance was analyzed using high performance liquid chromatography (HPLC) showed an absorption ability with an optimum contracting time of 180 minutes at a mass of 0,20 grams. The relative levels of absorbed asiaticoside based on the height-area of the peak area of the chromatogram for MIPL-D were 22,80%-19,65%; for MIPL-M in the amount of 12,58%-13,80%; and for NIP it is 31,23%-27,16%.