PRODUKSI FRUKTOSA DARI GLUKOSA MENGGUNAKAN ENZIM GLUKOSA ISOMERASE YANG DIHASILKAN DARI SUBMERGED FERMENTATION PADA SUBSTRAT KULIT JAGUNG OLEH STREPTOMYCES OLIVOCHROMOGENES.

• Main Author: Ainul Latifa, Diah
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/68715
• Institution: Institut Teknologi Bandung

HFS (High Fructose Syrup) is a sweetener that is massively used in the food and beverage industry. HFS in the food and beverage industry is used in the manufacture of carbonated beverages, canned fruit, jams and jellies, cakes, and dairy products. HFS is relatively stable at low pH, easier to handle, and gives baked goods a beautiful color. Generally, HFS is made from starch or cellulose which is less expensive than sucrose such as cassava starch. To produce HFS, cassava starch needs to be hydrolyzed to glucose and then isomerized to fructose. The process of isomerization of glucose into fructose can be catalyzed by the enzyme glucose isomerase or chemical catalysts. Isomerization with glucose isomerase is more environmentally friendly and the reaction occurs more specifically. This study aimed to determine the activity of the crude glucose isomerase enzyme produced by submerged fermentation using Streptomyces olivochromogenes and the production of fructose from glucose and to determine the kinetic parameters of the enzyme using an enzyme produced using an additional (supplement) carbon source of corn husks at a concentration of 1.5; 2; 2.5; 3% and at fermentation times of 24, 48, 72, and 96 hours. The highest activity of the glucose isomerase enzyme was found at a fermentation time of 96 hours with a value of 4.2 U/mL for extracellular enzymes with variations in corn husk concentration of 2% and 3.92 U/mL for intracellular enzymes with variations in corn husk concentrations of 1.5%. The highest isomerization conversion value was 22.9% by extracellular enzymes with 2% corn husk concentration variation for 96 hours. The Km and Vmax values obtained from the Lineweaver-Burk equation are 2.15 M and 10.15 U/mL.