FRAKSINASI DAN UJI AKTIVITAS ANTIJAMUR METABOLIT SEKUNDER JAMUR ASAL LAUT ASPERGILLUS FLAVUS

• Main Author: Wida Sari, Firda
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/69324
• Institution: Institut Teknologi Bandung

Fungal infectious diseases are one of the infections with a high prevalence rate. However, currently the availability of antifungal drugs is limited, and their effectiveness is reduced due to resistance problems. Therefore, it is necessary to constantly search for new antifungal drugs from natural sources. This study aimed to test the antifungal activity of secondary metabolites of the marine fungus Aspergillus flavus isolated from the mangrove roots in Sei Sembilang Village, North Sumatra, Indonesia. The research method is by fermenting the marine fungus Aspergillus flavus for 30 days to obtain the secondary metabolite of Aspergillus flavus. The fermented media was extracted using the liquid-liquid extraction method with ethyl acetate solvent, while the fermented mycelium was extracted using the maceration method with ethyl acetate solvent. The mycelium and media extracts obtained were tested for antifungal activity against Candida albicans using the disc diffusion method and microdilution method. The media extract has antifungal activity with a MIC ? ??± ?» ????ã?????íð?± ?í± ?±¸ð ±??? ®?í ??? ??ð»??ã ? ®?ð?ð???í± ?±¸ð ±??? ®?? ? fractionated using vacuum liquid chromatography method and tested the antifungal activity of the fraction against Candida albicans. Fraction B has the best antifungal activity with a MIC value of 125 ?ã????í± ?±???????ãã±???í ?»? ®?ð??????í ???¸±? ?± ??ð»??ã ? ®?ð?ð?? ?¸ í ??í± ???±??ð ? for further research.