FORMULATION OF PICKERING NANOEMULSION OF SOURSOP (ANNONA MURICATA L.) LEAF EXTRACT USING CITRULLINE AS CO-STABILIZER

• Main Author: Hidayati, Nurul
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/71097
• Institution: Institut Teknologi Bandung

Hyperpigmentation, one of the most common skin diseases, has high prevalence in Indonesia and affects all skin tones. Soursop leaf, an Indonesian herb, has the potential to be used as an antihyperpigmentation treatment. Soursop leaves have been used in a variety of topical treatments, such as emulsion. Emulsions, which contain immiscible two phase tend to form instability like coalesens. This research was done to create a Pickering nanoemulsion soursop leaf extract with citrulline as co-stabilizer. The soursop leaf first extracted with 96% ethanol, then added hot distilled water to remove chlorophyll from the extract. The simplicia and extracts were evaluated in terms of quality and quantity. The formulation of Pickering nanoemulsion was carried out by examining the system's stability using citrulline as co-stabilizer. Five cycles of freeze-thaw procedure were used for the stability test. To create the best preparations, it was also necessary to optimize the preparations' pH and humectants. The best preparation was then characterized and its antioxidant activity assessed using DPPH method. A yield of 12.74% was produced from soursop leaf extract. Based on paired t-test statistics, the Pickering nanoemulsion was more stable (significantly different) in the citrulline preparation. The preparation's pH has an impact on its stability since it changes the shape of citrulline. The Pickering nanoemulsion system's globule size and particle density are influenced by the type and existence in phase of humectants. Droplet size of the Pickering nanoemulsion was 23.2±8 nm and the colloidal network size was 350.9±87.5 nm. The Pickering nanoemulsion soursop leaf extract’s antioxidant activity (IC50 44.18±0.96 ?g/mL) was higher than that of soursop leaf extract (IC50 66.15±2.92 ?g/mL) with p-value<0,05.