IDENTIFICATION OF BOVINE GELATIN AND PORCINE GELATIN USING ATTENUATED TOTAL REFLECTANCE-FOURIER TRANSFORM INFRARED (ATR-FTIR) SPECTROSCOPY

• Main Author: Nilasari Azis, Suci
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/71103
• Institution: Institut Teknologi Bandung

Gelatin is a protein compound derived from the extraction process of animal bones or skin, such as cattle, pigs, fish or poultry. Gelatin is widely used in the industrial sector, both in food and medicine. The many benefits obtained from gelatin have increased public interest in using it. However, there are also increasing concerns regarding the type of animal it comes from, especially pigs which are not halal for Muslims. Thus, this study aims to identify bovine and porcine gelatin in gelatin raw material samples and capsule shells on the market. Identification was carried out using ATR-FTIR spectroscopy in the wave number range 4000-600 cm-1, in the wave number range 1700-1000 cm-1 using the principle component analysis (PCA) method (ATR-FTIR/PCA) and derivative spectroscopy (ATRFTIR/ derivative). The resulting IR spectra were compared to the IR spectra of bovine gelatin and porcine gelatin. Analysis of bovine and porcine gelatin in gelatin raw materials using PCA resulted in score plot graphs showing different clustered tendency areas. Analysis using derivative ATR-FTIR spectroscopy produced different gradient patterns at zero-crossing wave numbers: 1405.85 cm-1, 1410.11 cm-1, 1134.74 cm-1 and 1143.29 cm-1. The results of the gelatin raw material sample analysis showed the type of bovine gelatin. Thus, the ATR-FTIR/PCA and ATR-FTIR/derivative methods can be used for the identification of bovine and porcine gelatin in raw material samples.