CONVERSION OF CORN EXCTRACT INTO BIOETHANOL
The main issue of fuel’s availability in the world is the limited amount of raw material from fossils. Bioethanol is a potential alternative to replace fuel, it is ethanol produced from microorganism assisted biomass fermentation. Polysaccharide, in form of cellulose or amylum, is the raw material...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/71535 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The main issue of fuel’s availability in the world is the limited amount of raw material from fossils.
Bioethanol is a potential alternative to replace fuel, it is ethanol produced from microorganism assisted
biomass fermentation. Polysaccharide, in form of cellulose or amylum, is the raw material used to
produce bioethanol. Producing bio ethanol from corn exctract using amylase and glucoamilase from
sweet corn was performed in this research. To produce glucose, corn extract was hydrolysed with
assistance of ?-amylase. Assisted by Saccharomyces cerevisiae, glucose then fermented to produce
bioethanol. The objective of the research was to determine the optimum condition for the hydrolysis
process of corn extract which assisted by ?-amylase, then to perform glucose fermentation at the
optimum condition. The optimum concentration for the hydrolysis of corn extract, using 0.3 g/mL of
?-amylase’s concentration, was 0.9 g/mL and the optimum incubation was 90 minutes at 350C
temperature. The fermentation of glucose which produce by corn exctract hydrolysis assisted by
Saccharomyces cerevisiae at optimum condition, produced 13,591 % v/v of bioethanol. Means each 1
mmol of sweet corn extract can be converted into 2,47 % v/v of bioethanol at optimum hydrolysis
condition. |
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