ISOLASI, PEMURNIAN DAN KARAKTERISASI LIPASE TERMOSTABIL DARI BAKTERI TERMOFILIK ISOLAT LOKAL

• Main Author: Resdia Megasari, Citra
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/71760
• Institution: Institut Teknologi Bandung

Lipase (E.C 3.1.1.3, triacylglycerol hydrolase) is an important enzyme in biotechnological industries since a lot of applications in food, dairy, detergent, pulp, and pharmaceutical industries. Thermophilic microorganism, Geobacillus vulgivagus dYTae-14, isolated from hot spring in Indonesia, showed an extracellular lipase activity on lipid substrates at high temperature. On olive oil (1.5%, w/v) as a sole carbon source, the isolate dYTae-14 grew very rapidly at 70ºC and showed maximum lipase activity at late exponential phase, the activity slowly decrease up to stationary phase. The excreted lipase of dYTae-14 was partially purified by ammonium sulfate fractionation to give 4 fractions, 0-30%, 30-80%, 80- 100%, and 100% fractions. Fraction of 0-30% showed the highest activity by using paranitrophenyl palmitate as substrate. The partial purified enzyme showed optimal activity at 65ºC and pH 8.