OPTIMASI PRODUKSI ASAM LEMAK DARI MIKROALGA LAUT NANNOCHLOROPSIS
Microalgae produce vegetable oils that can be used as renewable fuel source. The object of the research is to optimize fatty acids production from marine microalgae Nannochloropsis sp. To produce biomass, the microalgae were cultivated through batch culture with aeration using air or CO2. Prior t...
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| Format: | Final Project |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/71773 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Microalgae produce vegetable oils that can be used as renewable fuel source. The
object of the research is to optimize fatty acids production from marine microalgae
Nannochloropsis sp. To produce biomass, the microalgae were cultivated through
batch culture with aeration using air or CO2. Prior to lipid extraction, microalgae
cells were pretreated using ultrasonic method or freeze-drying. The lipid content was
extracted in soxhlet apparatus using organic solvents of hexane or
chloroform:methanol (1:2). The fatty acid contents were characterized by the GC-MS
analysis. The biomass concentration obtained from cultivating with aeration using air
and CO2 was 2.63 and 0.32 g/L, respectively. The total of fatty acid content obtained
from cell cultivated with aeration using air and CO2; from cell preteated by
sonication and freeze-drying; and from lipid extraction using hexane and
chloroform:methanol (1:2) was 65.5 and 53.9%; 65.5 and 30.5%; and 29.9 and
30,5%, respectively. The results indicate that the optimum condition for fatty acids
production occur when cells are cultivated with aeration using air and pretreated by
sonication prior to extract their fatty acid using chloroform:methanol (1:2) solvent. |
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