KARAKTERISASI YTAE-13, ISOLAT BAKTERI PENGHASIL LIPASE DARI SUMBER AIR PANAS GEDONGSONGO
Selection result toward two Gedongsongo hot springs bacteria showed that YTae-13 was more potensial bacteria for futher research. When compared to other isolate, YTae-13 had higher amount of colony when it was grown in agar medium and had wider clear zone diameter when it was cultivated in lipase...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/71807 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Selection result toward two Gedongsongo hot springs bacteria showed that YTae-13 was
more potensial bacteria for futher research. When compared to other isolate, YTae-13 had
higher amount of colony when it was grown in agar medium and had wider clear zone
diameter when it was cultivated in lipase’s qualitative assay medium containing CaCl2 and
tween 20. Previous 16S rRNA analysis result, showed that this bacteria has closest homology
with Geobacillus lituanicus BGSCW9A89 which was confirmed to the results obtained from
this research. Morphologically, this bacteria has a small size colony, concentric growth,
convex surface, light yellow color, rod shaped-cell, and characterised as a gram positive
bacteria. Growth curve character from 7,0 x 107 CFU/mL starter for a period of 21 hour
showed two stationary phase at 11 and 17 hours after cultivation with growth rate of 0.153
h-1 and 0.0576 h-1 respectively. The comparison of growth-curve and enzyme activity curve,
showed that three peaks of enzyme were observed at 9, 15 and 19 hour-old cells whose
activity were 0.002566 unit/?g protein, 0.004164 unit/?g protein, and 0.005978 unit/?g
protein. |
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