STANDARDIZATION AND ANTIBACTERIAL ACTIVITY TEST OF ESSENTIAL OILS OF PATCHOULI (POGOSTEMON CABLIN) AND LAVENDER (LAVANDULA ANGUSTIFOLIA) AGAINST STAPYLOCOCCUS EPIDERMIDIS AND STREPTOCOCCUS MUTANS

• Main Author: Hafizha Hanun, Aqila
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/73567
• Institution: Institut Teknologi Bandung

Essential oil is one of the secondary metabolites of plants in the form of a mixture of volatile organic compounds that contribute to the taste and aroma of the plants. It is one of the commodities with high export value in Indonesia. It is widely used in traditional medicine. This study aims to standardize and determine the potential of patchouli (Pogostemon cablin) and lavender (Lavandula angustifolia) essential oils to inhibit bacteria that cause skin and dental problems, Staphylococcus epidermidis and Streptococcus mutans. Standardization parameters were organoleptic, refractive index, solubility in ethanol, acid number, ester number, specific gravity, and chemical content analysis using Gas Chromatography Flame Ionization Detector (GC-FID). Patchouli and lavender essential oil samples met the specifications of SNI 2006 and ISO 3515:2002. The antibacterial activity test was carried out qualitatively using the disc diffusion method while using Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) values using the microdilution method. The MIC values for Streptococcus mutans ATCC 25175 from patchouli and lavender oils were 0.625 and 10% (%v/v), respectively, while the MIC values for Staphylococcus epidermidis ATCC 12228 from patchouli and lavender oils were 0.3125 and 2.5% (%v/v), respectively. The MBC values for Streptococcus mutans ATCC 25175 from patchouli and lavender oils were 2.5 and 10% (%v/v), respectively, while the MBC values for Staphylococcus epidermidis ATCC 12228 from patchouli and lavender oils were 1.25 and 2,5% (%v/v), respectively. Based on these results, patchouli and lavender oils have more significant potential to inhibit and kill Staphylococcus epidermidis than Streptococcus mutans.