ANTIOXIDANT ACTIVITY OF CASSAVA (MANIHOT ESCULENTA CRANTZ) LEAVES, FLESH, AND PEEL EXTRACT

• Main Author: Theorisen, Natasha
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/73707
• Institution: Institut Teknologi Bandung

Free radicals are molecules having one or more unpaired electrons in their outer orbitals, which causes the compound to be very reactive. Free radicals are able to oxidize molecules and therefore may cause degenerative diseases. The oxidation of these molecules can be inhibited by antioxidants. One of the plants that has antioxidants is cassava. This study aims to determine the antioxidant activity of the extracts of leaves, tuber, and peel of cassava using the DPPH and CUPRAC methods, total phenolic and flavonoid content, the correlation between total phenols and flavonoids with antioxidant activity, the correlation of antioxidant activity between the two methods, and the identification and determination of flavonoid compound in selected extract. Extraction was performed by reflux using n-hexane, ethyl acetate and ethanol. The antioxidant activities of DPPH, CUPRAC, total phenols and flavonoids were determined by UV-visible spectrophotometry. Identification and quantification of flavonoid compound were determined by high performance liquid chromatography. Antioxidant activity of cassava extract with DPPH in the range of 1.309 - 68.182 mg AEAC/g and CUPRAC 2.095 - 68.050 mg AEAC/g. The highest total phenol was obtained from the ethanol leaves extract 11.133 ± 0.423 g GAE/100 g and the highest total flavonoid was obtained from the n-hexane peel extract 7.526 ± 0.526 g QE/100 g. In general, phenol and flavonoid compound greatly contributed to the antioxidant activity of cassava extract using the DPPH and CUPRAC methods. Both methods gave linear results on the antioxidant activity of cassava extract. The ethanol extract of cassava leaves contained rutin with a concentration of 0.863%. Leaves and peel of cassava had potency to be developed as antioxidant sources.