FORMULATION AND CHARACTERIZATION OF SOURSOP LEAF EXTRACT (ANNONA MURICATA L.) NANOEMULSION AND IN VITRO XANTHINE OXIDASE INHIBITORY ACTIVITY ASSAY
Gout is a metabolic disease that is induced by hyperuricemia condition which causes painful inflammatory in one or more joints of the body. This disease is caused by the precipitation of monosodium urate crystal which interacts with tissue during purine catabolism by xanthine oxidase (XO) enzy...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/73710 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Gout is a metabolic disease that is induced by hyperuricemia condition which causes painful
inflammatory in one or more joints of the body. This disease is caused by the precipitation of
monosodium urate crystal which interacts with tissue during purine catabolism by xanthine oxidase
(XO) enzyme. In the previous research, it was found that soursop leaf extract (Annona muricata L.)
has XO enzyme inhibitory activities even though it is very weak. Hence, in order to escalate the
inhibitory activity of XO enzyme, soursop leaf extract (EDS) was formulated into nanoemulsion of
soursop leaf extract (NE-EDS). The formulation process was performed by screening the solubility
of oil and co-surfactant optimization. Then, the process optimization of sonication time and formula
optimization using Box-Behnken Design Experiment were completed. The optimal formula
consisted of soursop leaf extract 0.1% (w/v), oleic acid 3% (w/v), tween 80 6% (w/v), colliphor RH40
2% (w/v), PEG 400 2% (w/v), and aquadest 86.9% (w/v). Characterization and evaluation of
organoleptic, particle size, polydispersity index, zeta potential, encapsulation efficiency,
morphology, and pH of NE-EDS were fulfilled. NE-EDS has a transparent, a bit green with no odour
organoleptic with a spherical morphology, particle size of 66.32 ± 4.43 nm, polydispersity index of
0.386 ± 0.026, zeta potential of 24.78 mV, encapsulation efficiency of 96.979 ± 0.863 %, and pH
5.18 ± 0.13. Stability study of 1 month shows that almost all NE formulations were relatively stable
when stored at 4, 25 and 40?. XO enzyme inhibitory activity assay shows an activity increase of
NE-EDS formula from IC50 EDS 469,400 ± 2.022 ppm into IC50 NE-EDS 34,109 ± 0,367 ppm. IC50
NE-EDS value shows an increase of 14 times of IC50 EDS value.
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