EFFECT OF ROTATION AMOUNT AND INOCULUM AMOUNT IN CITRIC ACID PRODUCTION USING SOLID STATE FERMENTATION IN DRUM BIOREACTOR

• Main Author: Wijayanto, Tito
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/73741
• Institution: Institut Teknologi Bandung

Citric acid has become a high-needs commodity nationally with an industrial market quantity of 2.39 million tons and a compound annual growth rate of 4% in the 2022-2027 period. This growth is due to the widespread application of citric acid in the food industry, beverages, cleaners and detergents, as well as the pharmaceutical industry. Currently, commercial production of citric acid is still commonly carried out through the submerged fermentation (SmF) method. However, SmF production of citric acid consumes high water and energy, increases the potential for contamination and wastewater. The SSF process is currently being developed to produce citric acid by utilizing agro-industrial waste such as cassava pulp using Aspergillus niger. In this study, the operating conditions varied were the amount of stirring and the concentration of inoculum in the bioreactor. This study is intended to analyze the yield of citric acid obtained from variations in operating conditions, then the results of the analysis will be used to determine the interaction between operating conditions that optimize citric acid yield by utilizing the analysis of variance (ANOVA) method and full factorial analysis on Minitab. The results of this study showed that the condition of stirring twice a day with an inoculum concentration of 5% was the operating condition that produced the highest citric acid concentration, which was 0.0051 g citric acid / g substrate. When analyzed using ANOVA, it was found that the factors that gave significant results to citric acid yield were only stirring conditions, while the inoculum concentration factor and the interaction between stirring and inoculum concentration were not significant for citric acid yield.