EFFECTS OF 2,4-DICHLOROPHENOXYACETIC ACID AND 6-BENZYLAMINOPURINE ON CELL SUSUPENSION CULTURE OF AQUILARIA MALACCENSIS LAM.

• Main Author: Mubina Adfinda, Fathya
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/75054
• Institution: Institut Teknologi Bandung

<p align="justify">to poaching. One effort that can be done to conserve A. malaccensis is propagation through in vitro culture techniques which can be done by developing callus cultures and cell suspensions. This study aims to determine the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (BAP) to induce callus culture and cell suspension of A. malaccensis. Callus initiated from 1 year old young shoots of A. malaccensis on solid Murashige Skoog (MS) medium with the addition of 0.3; 0.4; and 0.5 ppm 2,4-D and BAP with a concentration of 1, 1,5; and 2.0 ppm. The results showed that a combination of 0.5 ppm 2,4-D and 2.0 ppm BAP and a combination of 0.4 ppm 2,4-D and 1.5 ppm BAP produced the best response in inducing callus formation. Then the callus was subcultured into liquid MS medium to induce the formation of a cell suspension culture. Quantitative parameters in the form of cell concentrations were observed weekly for a period of 4 weeks to observe the growth curve, doubling time, and specific growth rate. The combination of 0.4 ppm 2,4-D and 1.5 ppm BAP was able to induce the formation of a cell suspension culture with a specific growth rate of 0.062 and a doubling time of 11 days, while the combination of 0.5 ppm 2,4-D and 2.0 ppm BAP was able to induce the formation of a cell suspension culture with a specific growth rate of 0.031 and a doubling time of 22 days. Observation of cell morphology showed that the suspension culture on a combination of 0.4 ppm 2,4-D and 1.5 ppm BAP was dominated by single cells with a percentage of rounded cells of 91.67%; and the percentage of oval cells was 8.33%, whereas in the combination of 0.5 ppm 2,4-D and 2.0 ppm BAP, suspended cells were dominated by single cells with a percentage of round cells 89.65%; and the percentage of oval cells is 10.35%. The combination of 0.5 ppm 2,4-D and 2.0 ppm BAP stimulated more cell growth in the 4th week but with a lower percentage of rounded cells than the 0.4 ppm 2,4-D combination and 1.5 ppm BAP. It can be concluded that the combination of 0.5 ppm 2,4-D and 1.5 ppm BAP can induce faster multiplication of A. malaccensis cell suspension cultures than the combination of hormones 0.5 ppm 2,4-D and 2.0 ppm BAP. Fathya Mubina Adfinda (10618025)