BIOSYNTHESIS OF ANTIBACTERIAL ZNO NANOPARTICLES FROM CINNAMOMUM BURMANNII
<p align="justify">Overuse of antibiotics leads to the development of antibiotic resistance. To counter this issue, the development of natural antimicrobial materials is crucial. Examples of such materials include chitosan and natural rubber. Nanoparticles also serve as effective...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/76009 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | <p align="justify">Overuse of antibiotics leads to the development of antibiotic resistance. To counter
this issue, the development of natural antimicrobial materials is crucial. Examples
of such materials include chitosan and natural rubber. Nanoparticles also serve as
effective antimicrobial agents. One method of nanoparticle production is through
a green approach, utilizing plant extracts in a process called biosynthesis. This
method offers the advantage of avoiding toxic chemicals and high-energy
equipment. Notably, cinnamon has been extensively investigated and demonstrates
notable antimicrobial qualities.
This research aims to investigate the properties of antimicrobial zinc oxide (ZnO)
nanoparticles derived from cinnamon. The study explores different experimental
variables, including synthesis temperatures (70°C and 95°C) and extract ratios
(30% and 70%). Calcination is carried out at 650°C. The nanoparticles are
characterized using techniques such as FTIR, XRD, TEM, and DLS to analyze
functional groups, crystal structure, particle size, and size distribution.
Additionally, the antibacterial activity is assessed using the total plate count
method with Staphylococcus aureus bacteria.
The initial synthesis results prior to the calcination process do not exhibit the
formation of ZnO nanoparticles. At a calcination temperature of 650°C, ZnO
exhibits peak absorption at a wave number of 435-457 cm-1. The diffraction pattern
indicates a hexagonal crystal structure. Particle size analysis yields dimensions
ranging from 17 to 49 nm, with instances of agglomeration. The intensity and
degree of XRD crystallinity correlate with synthesis temperature and extract ratio.
Antibacterial tests using Staphylococcus aureus bacteria demonstrate that ZnO
nanoparticles synthesized at a concentration of 97 ppm inhibit bacterial colony
growth by 68-94%.
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