BIOSYNTHESIS OF ANTIBACTERIAL ZNO NANOPARTICLES FROM CINNAMOMUM BURMANNII

• Main Author: Ratnasari, Ayu
• Format: Theses
• Language: Indonesia
• Subjects: Teknik kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/76009
• Institution: Institut Teknologi Bandung

<p align="justify">Overuse of antibiotics leads to the development of antibiotic resistance. To counter this issue, the development of natural antimicrobial materials is crucial. Examples of such materials include chitosan and natural rubber. Nanoparticles also serve as effective antimicrobial agents. One method of nanoparticle production is through a green approach, utilizing plant extracts in a process called biosynthesis. This method offers the advantage of avoiding toxic chemicals and high-energy equipment. Notably, cinnamon has been extensively investigated and demonstrates notable antimicrobial qualities. This research aims to investigate the properties of antimicrobial zinc oxide (ZnO) nanoparticles derived from cinnamon. The study explores different experimental variables, including synthesis temperatures (70°C and 95°C) and extract ratios (30% and 70%). Calcination is carried out at 650°C. The nanoparticles are characterized using techniques such as FTIR, XRD, TEM, and DLS to analyze functional groups, crystal structure, particle size, and size distribution. Additionally, the antibacterial activity is assessed using the total plate count method with Staphylococcus aureus bacteria. The initial synthesis results prior to the calcination process do not exhibit the formation of ZnO nanoparticles. At a calcination temperature of 650°C, ZnO exhibits peak absorption at a wave number of 435-457 cm-1. The diffraction pattern indicates a hexagonal crystal structure. Particle size analysis yields dimensions ranging from 17 to 49 nm, with instances of agglomeration. The intensity and degree of XRD crystallinity correlate with synthesis temperature and extract ratio. Antibacterial tests using Staphylococcus aureus bacteria demonstrate that ZnO nanoparticles synthesized at a concentration of 97 ppm inhibit bacterial colony growth by 68-94%.