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This study was to determine the optimum condition of enzymatic degradation of quinine, cinchonine, and cinchonidine using rat hepatic homogenate suspension. The concentration of quinine, cinchonine, and cinchonidine was determined spectrophotometrically at 280, 300, and 315 nm respectively. Enzymati...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/7623 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | This study was to determine the optimum condition of enzymatic degradation of quinine, cinchonine, and cinchonidine using rat hepatic homogenate suspension. The concentration of quinine, cinchonine, and cinchonidine was determined spectrophotometrically at 280, 300, and 315 nm respectively. Enzymatic degradation of quinine, cinchonine, and cinchonidine at concentration level of 0.9 - 2.5 mg/ml was monitored after 30, 60, 90, and 120 minutes of incubation time. The degradation level of quinine, cinchonine, and cinchonidine were respectively found to be 11.5, 12.17, and 18.63 % after 30 minutes incubation time; 45.06, 8.3, and 13.67 % after 60 minutes incubation time; 30.33, 8.5, and 7,74 % after 90 minutes incubation time, as well as 13.01, 5.37, and 1.77 % after 120 minutes incubation time, calculated as procentage of theoretical concentration. |
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