VALIDATION OF SUCRALOSE DETERMINATION METHODS IN SWEETENER PRODUCTS AND ESTIMATION OF SUCRALOSE EXPOSURE IN DIABETIC PATIENTS AT KIARA HUSADA CLINIC BANDUNG

• Main Author: Zhafran Octonariz, Vito
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/76565
• Institution: Institut Teknologi Bandung

Food additives (FA) are substances added to food with the aim of influencing the nature or form of food. One of the FA of the sweetener function class permitted to be used in several food categories is sucralose. Table top sweetener is a product that may contain sucralose and is commonly consumed by diabetic patients. This study aims to obtain a valid method for sucralose determination in a table top sweetener, data on sucralose levels in the sample, and estimation of sucralose exposure in diabetic patients at the Kiara Husada Clinic, Bandung. Determination of sucralose was conducted by means of high-performance liquid chromatography (HPLC) method and the validation parameters determined include linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The precision and accuracy of the method are expressed as relative standard deviation (RSD) and percent recovery of the standard addition determination in the range of 80-120% of the actual concentration of sucralose in the sample. The LOD and LOQ of the methods were obtained statistically from the calibration curve. Exposure assessment were carried out by filling out questionnaires by diabetic patients as respondents using the food recall and food frequency questionnaire method. From the results, the calibration curve with the equation Y = 219.91x – 2535 and R2 value 0.999 was obtained, RSD was in the ranged of 1.06 – 3.08%, while percent of recovery was in the range of 98.36 – 103.88%, and LOD and LOQ of the method were respectively 9.48 and 28 .73µg/mL. Average levels of sucralose in products A and B were 4.83 and 10.57 mg/g, respectively Exposure to sucralose from the two products in the ranged of 0.02 – 0.27 mg/kg body weight/day, equivalent to 0.16 – 1.83% of the acceptable daily intake (ADI) of sucralose of 15 mg/kb body weight/day. Based on the overall results obtained, it could be concluded that the sucralose method determination in table top sweetener products was successfully obtained, the average level of sucralose in the product were 4.83 and 10.57, and exposure to sucralose in diabetic patients did not exceed the ADI value.