PRODUKSI BIOETANOL DARI LIMBAH BATANG PISANG MELALUI OPTIMALISASI PROSES \TEXTIT{SIMULTANEOUS SACCHARIFICATION AND FERMENTATION} (SSF) DENGAN \TEXTIT{SACCHAROMYCES CEREVISIAE}

• Main Author: Gunawan, Agung
• Format: Theses
• Language: Indonesia
• Subjects: Teknologi
• Online Access: https://digilib.itb.ac.id/gdl/view/77432
• Institution: Institut Teknologi Bandung

Indonesia is one of the highest banana commodity countries in the world. Bananas are favoured by many people because planting Banana stem processing is one of the problems related to agricultural waste, because there is still a lack of banana stem processing technology. The solution to the banana stem waste problem is to process it into bioethanol. This study aims to determine the optimisation of banana stem with steam treatment and Simultaneous Saccharification and Fermentation process with Saccharomyces cerevisiae from banana stem waste so as to produce bioethanol production with the highest concentration. The optimisation process with steam treatment is part of the stages for the delignification compounds. Simultaneous Saccharification and Fermentation process has advantages in bioethanol production and effectiveness of relatively short production process by applying the combination of enzymatic hydrolysis and fermentation simultaneously. Enzymatic hydrolysis uses cellulase and xylanase enzymes. The fermentation process uses Saccharomyces cerevisiae which is useful as a biocatalyst in converting glucose monomers into bioethanol. This study used Taguchi method experimental design with Orthogonal array design L9(34) which uses four factors (enzyme, sieve pore size, temperature and pH) and three levels of each factor (enzyme concentration: 1%; 2.5%; 5% (v/v); sieve pore size: 40 mesh, 50 mesh, 60 mesh; temperature: 30oC, 35oC, 40oC; and pH: 5.00; 6.00; 7.00) with the determination of the larger is better category to get the highest bioethanol production. The results showed that the factors that affect the quality of the highest bioethanol production are enzyme concentration and sieve pore size (mesh). While the optimal conditions for bioethanol production proposed based on the results of the study obtained optimal bioethanol production with the selected factor level of 5% (v/v) enzyme concentration, 60 mesh sieve pore size, temperature 35oC, and pH 5.00. The highest bioethanol production obtained was 9.0 g/L.