EFFECT OF LAVENDER EXTRACT, ROSE OIL, AND RICE MILK TOWARDS COL1A1 AND ELN GENE EXPRESSION ON NIH/3T3 CELLS

• Main Author: Marchia Henly, Calista
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78372
• Institution: Institut Teknologi Bandung

Skin integrity is needed so the skin can perform its functions optimally as the human body's first protection towards pathogens, UV light, and injuries that happen chemically or mechanically. Rose oil, rice milk, and lavender extracts have potential to increase skin integrity indirectly because of the antioxidant compounds found in it. The purpose of this research is to explore rose oil, rice milk, and lavender extracts in increasing skin integrity. In this research, gene expression analysis is done towards genes in NIH/3T3 cells that have a role in skin integrity, such as type I collagen (COL1A1) and elastin (ELN) using quantitative real-time PCR (qPCR). Cell viability assay towards NIH/3T3 cells was done as a preliminary test to prove that every sample concentration used is nontoxic. According to qPCR results, rose oil and lavender extracts show an increase of COL1A1 gene expression in NIH/3T3 cells. ELN gene expression analysis couldn't be done because of bad primer efficiency. Primer screening and analysis for gene expression analysis of genes expressed by keratinocytes that have a role in skin integrity, such as filaggrin (FLG), involucrin (IVL), and loricrin (LOR), was also done in this research. This research shows that rose oil and lavender extracts have potential to increase skin integrity.