ISOLATION AND ANTIFUNGAL ACTIVITY TEST OF SECONDARY METABOLITES MARINE FUNGUS ASPERGILLUS FLAVUS

• Main Author: Nur Azmi, Raihan
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78446
• Institution: Institut Teknologi Bandung

As a tropical country, Indonesia is a fertile region for fungi to grow, so that fungal infections often infect Indonesian citizen. The use of inappropriate antifungal drugs can increase the risk of antifungal resistance. Several attempts have been made to overcome antifungal resistance problem, one of them is to isolate antifungal compounds with better antifungal activity so that they can be used as active ingredients candidate in the future. A fraction isolated from marine fungi Aspergillus flavus has been shown to have activity against Candida albicans. This research aimed to determine the antifungal activity of fractions, subfractions, and isolate candidate from marine fungi Aspergillus flavus, determine their characteristics, and isolates the target compound. The preliminary screening test for the antifungal activity of fractions against Candida albicans was carried out using disc diffusion method. The inhibition diameter of fraction was 18,97 ? 1,41 mm. The sample fraction was sub fractionated by flash preparative chromatography with a mix of methanol-water as a mobile phase and C-18 column as a stationary phase. Each subfractions was tested for its antifungal activity against Candida albicans by microdilution method. Subfraction A, subfraction B, subfraction C, and subfraction-waste had antifungal activity with MIC values of 128 ?g/mL. Densitometry TLC analysis of selected subfractions showed that the dominant compound had Rf 0,66 (candidate isolate 4) with %area value of 81,54% and maximum wavelength of 325 nm. TLC profile analysis of the compounds was carried out with specific dyeing reagents citroboric, Liebermann-Burchard, and Dragendorff. The selected subfraction, subfraction B, was purified by preparative thin layer chromatography (TLC) with chloroform-methanol = 9,5:0,5 as mobile phase. The purifications result obtained several candidates, isolate 1, isolate 2, isolate 3, isolate 4, and isolate 5. Monitoring of the isolate candidates was carried out using TLC silica gel GF254 with chloroform-methanol = 9,5:0,5 as mobile phase which showed that isolate candidate 2 and isolate 5 already showed a single spot. It was found that subfraction B allegedly contained steroids/triterpenoids. Isolation of targeted compound from active fraction of marine fungi Aspergillus flavus has been successfully carried out, but its activity against Candida albicans has not been confirmed.