ISOLATION OF HETEROTROPHIC MICROALGAE FROM MANGROVE FALLEN (RHIZOPHORA APICULATA) AS SOURCE OF DOCOSAHEXAENOIC ACID (DHA)

• Main Author: Fathurohman, Mochamad
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/78952
• Institution: Institut Teknologi Bandung

Omega-3 fatty acids particularly docosahexaenoic acid (DHA) are classified as functional fatty acids that are very beneficial for health. All this time, the main source of DHA is obtained from fish which are extracted into fish oil. However, due to the limitation of purity, heavy metal contamination, consumer acceptance and production processes, the search for alternative source of DHA such as heterotrophic microalgae. This aims of this research is to carry out isolation and characterized of heterotrophic microalgae as source of docosahexaenoic acid (DHA) for Mangrove Areas at Cilacap. The heterotrophic microalgae were isolated from mangrove fallen leaves (Rhizophora apiculata) using direct planting method, The morfology of microalgae colony were observed through light microscope and then it was purified to produce single colonies that is subsequently fermented for 14 day to produce biomass. Fatty acids were extracted and methylated through direct transesterification method. The content of DHA by was using determination Gas Chromatography. The result were four isolates heterotrophic microalgae MTKCI, MTKC2, MTKC3 and MTKC4. The isolate of MTKC2 which only detected produced DHA with value of The MTKC2 was subsequently were identified and characterized by molecular biology. The identification results showed that MTKC2 include genus Thraustochytrium.