EFEK ANTIGLIKASI IN VITRO BEBERAPA EKSTRAK TUMBUHAN OBAT INDONESIA DAN ISOLASI SENYAWA TUMBUHAN TERPILIH DALAM PENANGANAN DIABETES MELLITUS
Background and purpose: In the condition of hyperglycemia, excess glucose in the blood will cause glycation. Glycation or the Maillard reaction is also called non-enzymatic reaction between the amine group -NH2 (lysine and arginine residues) of blood proteins with carbonyl groups of glucose. The...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/79016 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Background and purpose: In the condition of hyperglycemia, excess glucose in
the blood will cause glycation. Glycation or the Maillard reaction is also called
non-enzymatic reaction between the amine group -NH2 (lysine and arginine
residues) of blood proteins with carbonyl groups of glucose. The early stage of
glycation will form a Schiff base and Amadori product. Further reaction of
glycation will generate Advanced Glycated End Products (AGEs). AGEs are
compounds marker for some diseases, such as atherosclerosis, kidney failure,
Alzheimer's, or diabetes. Research about in vitro antiglycation effects against
several plant extracts with the aim for screening drugs of plants that have
antiglycation effect had been conducted. Plants used in this study were
traditionally used to lower blood glucose levels, included Cinnamomum burmanni
Ness ex.Bl., Andrographidis paniculata (Burm.f.) Ness, Phaleria macrocarpa
(Scheff) Buerl., Annona muricata Linn., Momordica charantia Linn., Mangifera
indica Linn. (Bapang), Mangifera indica Linn. (Arumanis), Myristica fragrans
Houtt., Tinosporae crispa (Linn.) Miers, Annacardium occidentale Linn.,green tea
(Camellia sinensis (L.) O. Kuntze), white tea (Camellia sinensis (L. ) O. Kuntze),
black tea (Camellia sinensis (L.) O. Kuntze), Vinca rosea Linn., Ocimum
bacillicum Linn., Orthosipon stamineus Benth., Garcinia mangostana Linn.,
Annona squomosa Linn., Psidium guajava Linn., Zingiberis officinale Roscoe.,
and Averrhoa bilimbi Linn. Methods: The research was conducted by inhibition
of plant extracts against albumin glycation reactions in vitro, by treating plant
extract samples with albumin and glucose, which will produce Hydroxy Methyl
Furfural (HMF), then absorbation measured using UV-Vis spectroscopy. Results:
The results showed that the extract with the antiglycation activity of more than
50% shown by extracts of Annona squomosa Linn. (65.76 ± 8.59%), extract of
Psidium guajava Linn. (54.52 ± 6.95%), and Avverhoa bilimbi Linn. (51.02 ±
9.35%). Psidium guajava Linn ethanol extract was fractionated by liquid-liquid
extraction method to obtain the fraction of ethyl acetate and water fractions. Ethyl
acetate fraction has higher antiglycation activity than waterfraction, 68.42 ±
5.43% compared with 33.98 ± 9.24% respectively. The results of the isolated were
then characterized using UV-Vis and NMR spectroscopy. The characterization
results showed that the isolate is quercetin. Conclusion : This study shows that
several extract medicinal plants have activity as antiglycation. One of extract that
have activity is Psidium guajava Linn. with inhibition of 54.52 ± 6.95 %. Ethyl
acetate fraction of Psidium guajava Linn. has the highest inhibitory antiglycation
than thte fraction of water.
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