EFEK ANTIGLIKASI IN VITRO BEBERAPA EKSTRAK TUMBUHAN OBAT INDONESIA DAN ISOLASI SENYAWA TUMBUHAN TERPILIH DALAM PENANGANAN DIABETES MELLITUS

• Main Author: Erika Herawati, Irma
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/79016
• Institution: Institut Teknologi Bandung

Background and purpose: In the condition of hyperglycemia, excess glucose in the blood will cause glycation. Glycation or the Maillard reaction is also called non-enzymatic reaction between the amine group -NH2 (lysine and arginine residues) of blood proteins with carbonyl groups of glucose. The early stage of glycation will form a Schiff base and Amadori product. Further reaction of glycation will generate Advanced Glycated End Products (AGEs). AGEs are compounds marker for some diseases, such as atherosclerosis, kidney failure, Alzheimer's, or diabetes. Research about in vitro antiglycation effects against several plant extracts with the aim for screening drugs of plants that have antiglycation effect had been conducted. Plants used in this study were traditionally used to lower blood glucose levels, included Cinnamomum burmanni Ness ex.Bl., Andrographidis paniculata (Burm.f.) Ness, Phaleria macrocarpa (Scheff) Buerl., Annona muricata Linn., Momordica charantia Linn., Mangifera indica Linn. (Bapang), Mangifera indica Linn. (Arumanis), Myristica fragrans Houtt., Tinosporae crispa (Linn.) Miers, Annacardium occidentale Linn.,green tea (Camellia sinensis (L.) O. Kuntze), white tea (Camellia sinensis (L. ) O. Kuntze), black tea (Camellia sinensis (L.) O. Kuntze), Vinca rosea Linn., Ocimum bacillicum Linn., Orthosipon stamineus Benth., Garcinia mangostana Linn., Annona squomosa Linn., Psidium guajava Linn., Zingiberis officinale Roscoe., and Averrhoa bilimbi Linn. Methods: The research was conducted by inhibition of plant extracts against albumin glycation reactions in vitro, by treating plant extract samples with albumin and glucose, which will produce Hydroxy Methyl Furfural (HMF), then absorbation measured using UV-Vis spectroscopy. Results: The results showed that the extract with the antiglycation activity of more than 50% shown by extracts of Annona squomosa Linn. (65.76 ± 8.59%), extract of Psidium guajava Linn. (54.52 ± 6.95%), and Avverhoa bilimbi Linn. (51.02 ± 9.35%). Psidium guajava Linn ethanol extract was fractionated by liquid-liquid extraction method to obtain the fraction of ethyl acetate and water fractions. Ethyl acetate fraction has higher antiglycation activity than waterfraction, 68.42 ± 5.43% compared with 33.98 ± 9.24% respectively. The results of the isolated were then characterized using UV-Vis and NMR spectroscopy. The characterization results showed that the isolate is quercetin. Conclusion : This study shows that several extract medicinal plants have activity as antiglycation. One of extract that have activity is Psidium guajava Linn. with inhibition of 54.52 ± 6.95 %. Ethyl acetate fraction of Psidium guajava Linn. has the highest inhibitory antiglycation than thte fraction of water.