VALIDASI DAN PENGEMBANGAN METODE ANALISIS PENENTUAN NEOTAM DALAM PRODUK JELI DAN MINUMAN RINGAN SECARA KROMATOGRAFI CAIR KINERJA TINGGI

• Main Author: Andriany, Dinny
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/79019
• Institution: Institut Teknologi Bandung

Background: Neotam is a high intense artificial sweetener with a sweetness level of 7.000 to 13.000 times sweeter than sucrose and 30 to 60 times sweeter than aspartame. An ADI (Acceptable Daily Intake) value of 0 - 2 mg / kg body weight [day is established by Joint FAO / WHO Committee for Food Additives (JECFA) and hence this sweetener belongs to food additive with low ADI value. From the point view of risk analysis, the exposure level of such food additive is of high priority to evaluated. For that purpose, an analytical method for determination of neotam in different food product is necessary. The aim of this study was to obtain an analytical method suitable for determination of neotame in jelly and drinks. Method: A high performance liquid chromatography method has been developed and validated for determination of neotam in jelly and drinks. A Shimadzu HPLC chromatograph instrument equipped with a Zorbax Eclipse Plus C 18 column (4.5 x 150 mm, 511m) and UV detector of 210 nm were applied. Mobile phase consist of a mixture of 0.05 M potassium dihidrogen phosphate buffer (pH 4.5) and acetonitrile (7 : 3). Flow rate was adjusted at 1.2 mL min- and column temperatue was maintained at 40 C. Samples were prepared by filtration and dissolved in the mobile phase. Result: Two calibration curves for determination of neotame in jelly and drinks were separately prepared. The first calibration curve with the regression equation of y — 29171.38x + 284.9728 was linear over the range of 2.085 - 12.057 ug/ml with the correlation coefficient of r 0.999962 and was applied for jelly samples, while the second one with the regression equation of y ¯ 29420.09x — 896.09 was linear over the range of 2.02 — 12.12 mg/L with the correlation coefficient of r 0.999818 and was-applied for drinks samples. The limits of detection and limits of quantification, calculated from the two curves were 0.14 and 0.42 gg/ml and 0.31 and 0.87 gg/ml, respectively. The precision of the method, stated as inter-day RSD, for jelly and drinks samples were 0.576 and 0.623% , respectively. The recoveries of neotam standard addition from jelly and drinks samples were 99.44 — 102.28% and 93 - 103%, respectively. Despite the presence of aspartame as related compound, the method showed good selectivity. Based on overall results, it was concluded that the proposed method has been succesfully developed and can be applied for determination of neotam in jelly and drinks samples.