DEVELOPMENT OF ANALYTICAL METHOD FOR DETERMINATION OF LEAD (PB) AND CADMIUM (CD) IN RICE APLLIYING ANALYTICAL TECHNIQUE OF GRAPHITE FURNACE ATOMIC ABSORPTION SPECTROFOMETRY

• Main Author: Noor Adrianto, Rizqie
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/79024
• Institution: Institut Teknologi Bandung

Heavy metals are hazard sources that may contaminate foods and hence probably cause health problems. Cadmium (Cd) and lead (Pb) are contaminants commonly reported to be present in foodstuffs. Rice is consumed as the main staple food in Indonesian and consequently its safety aspect should be assured to prevent health problems.- From the view of risk analysis, the quantities of Cd and Pb in rice should be determined to enable the quantitative prediction of exposure level of these heavy metals. For this purpose, suitable quantitative analytical method of Cd and Pb is needed. This study aims to obtain analytical method for determination of Pb and Cd in rice samples applying analytical technique of Atomic Absorption Spectrophotometry - Graphite Furnace (GF-AAS) and predicted data on exposure levels of Pb and Cd from rice samples commercially available in Bandung. Prior Pb and Cd determination, samples were prepared by means of wet destruction method using nitric acid (HN03) and hydrogen peroxide (H202) as reagent in a microwave digestion apparatus. The calibration curve of Pb with the regression equation of y 0.00834x + 0.00401 was linear in the concentration range of 10-60 ng / mL with a correlation coefficient of r 0.9999 while that of Cd with the regression equation of y 0.09554x + 0.00077 was linear in the concentration range of 1 - 6 ng / mL with a correlation coefficient of r=0.9999. Limit of detection and limit of quantitation calculated from the calibration curves were respectively 0.296 and 0.874 ng / mL for Pb, while those of Cd were 0.035 and 0.102 ng / mL. The precision of this method stated as interday RSD for Pb and Cd were 0.22 and 0.20%, respectively. The recoveries of standard additions ofPb and Cd were respectively 98.73 to 102.81 % and 98.16 to 99.95%. The concentrations of Pb and Cd in 8 rice samples were 89.12 — 145.18 ng/g for Pb and 14.13 — 26.22 ng/g for Cd and hence still fullfil the requirements stated by the regulation. Intake levels of Pb and Cd from rice were predicted in the range of 11.04 - 17.98% and 6.30 -11.69% of Tolerable Daily Intake (TDD, respectively. Based on the overall results, it was concluded that this method has been successfully developed and can be used for the determination of of Pb and Cd in rice samples and the exposure levels of both heavy metals from rice were less than 100% ofTD1.