ACTIVITY OF RECOMBINANT CYCLODEXTRIN GLYCOSYLTRANSFERASE ENZYME FROM BACILLUS SP. A2-5A WITH STARCH AND MALTODEXTRIN SUBSTRATES FROM PINEAPPLE STEM

• Main Author: Owen, Jonathan
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82557
• Institution: Institut Teknologi Bandung

The enzyme cyclodextrin glycosyltransferase (CGTase) can catalyze the formation of cyclodextrin (CD) with polysaccharide substrates. CD production is often focused on the ? form (CD-?). One effort to develop the CD-? production system is substrate optimization. In previous research, recombinant CGTase (rCGTase) from Bacillus sp. A2-5a was used, and its performance was characterized using sago starch (Metroxylon sagu). This study aims to investigate the activity of rCGTase on new candidate substrates to produce CD-?, specifically starch and maltodextrin from pineapple stems (Ananas comosus). Initial tests on the two substrates using modified phenolphthalein agar media showed the potential formation of CD-?. Further quantitative tests were conducted on the two substrates at a concentration of 0.5% with 0.02 UA pure rCGTase; temperature 55°C; pH 6; duration 30 minutes. Quantification based on the colorimetric method showed that the production of CD-? by pineapple stem starch was 52.706 µg/mL, while the use of maltodextrin substrate from pineapple stems required further measurement using High-Performance Liquid Chromatography (HPLC).