SYNTHESIS OF PROPYLAMINE MODIFIED SILICA AS A PACKING MATERIAL FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN

• Main Author: Salsabila, Rizki
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/82750
• Institution: Institut Teknologi Bandung

Silica can be synthesized through the sol-gel method. In general, silica is used as a column packing material or stationary phase in High Performance Liquid Chromatography (HPLC) methods. The success of analysis using KCKT depends on the choice of column (stationary phase) and the right working conditions. In this study, silica material was synthesized from tetraethylorthosilicate (TEOS) prekursor modified with propylamine active group derived from 3-aminopropyl triethoxysilane (APTES) compound using sol-gel method. The propylamine-modified synthesis results were characterized using DRS-FTIR and Scanning Electron Microscopy (SEM). Propylamine modified silica used as KCKT column was tested for its performance to separate sugar mixture solution. Based on FTIR data, it was found that there were new peaks at wavelengths of 1657 and 3403 cm-1 (N-H peak), 1381 and 2922 cm-1 (C-H peak), and 947 cm-1 (Si-C peak). All of these peaks are also found in the 3-APTES spectrum, so it can be concluded that silica modification using 3-APTES has been successfully carried out. Then based on SEM data, amine-modified silica is spherical and measures 1.72-2.02 µm. Modified silica was put into a chromatography column with methanol solvent eluent. The results of this study have been able to retain sugars other than maltose in the sugar mixture (fructose, glucose, and maltose) but the peaks are not optimally separated. The conditions used to retain sugar are acetonitrile:water eluent 100:0 (v/v) and a flow rate of 1.7 mL/min with the following order of separation: fructose-glucose.