SYNTHESIS OF PHENYLTRIETHOXYSILANE (PTES) MODIFIED SILICA FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN FILLING MATERIAL

• Main Author: Mardhiyah, Salsabila
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/82753
• Institution: Institut Teknologi Bandung

High Performance Liquid Chromatography (HPLC) has been applied in various fields such as pharmaceuticals, biotechnology, polymers and the food industry. One of the important components and the core of the HPLC system is the column. Column filling materials generally consist of modified silica with certain functional groups, such as Phenyltriethoxysilane (PTES). The PTES potential is used to modify silica to produce phenyl modified silica because this group can carry out ?-? interactions with the double bond electrons in the analyte. The aim of this research is to synthesize phenyl modified silica as a filler for HPLC columns. Silica was synthesized using the St?ber sol-gel method using tetraethylorthosilicate (TEOS) precursor, ethanol solvent and ammonia as a catalyst. Then, it was modified using PTES and isopropanol solvent. Based on the IR results, absorption peaks were obtained in the wave number range of 2918.3 cm-1 and 2850.79 cm-1 which showed C-H vibrations, absorption peaks of 1099.43 cm-1 which showed Si-O-Si vibrations, O-H vibrations at 3433, 29 cm-1, C=C vibration at 1637.56 cm-1 and C-O stretching vibration is shown at wave number 1382.96 cm-1. SEM characterization shows spherical silica with a size of 1-2 ?m. The phenyl silica column was successfully loaded and was able to separate paracetamol and caffeine under the eluent conditions MeOH:Water (20:80), flow rate 1 mL/minute, standard analyte concentration of 40 ppm, injector volume of 20 ?L and wavelength of 274 nm using a DAD detector. The resolution obtained was 4.005 and selectivity was 2.0341 with an R2 value of 0.9973 for paracetamol in the range of 0.5 ppm – 100 ppm and 0.9957 for caffeine in the range of 0.5 ppm – 100 ppm.