EVALUATION OF CORN PROLAMIN-BASED BIOPLASTIC MEMBRANES FOR PROTEIN PURIFICATION
Proteins have various uses in enhancing the quality and safety of products in the food industry. One type of protein widely used in the food industry is enzymes. The downstream process of enzyme production is enzyme purification. A common method used in enzyme purification is ultrafiltration memb...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/84433 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Proteins have various uses in enhancing the quality and safety of products in the
food industry. One type of protein widely used in the food industry is enzymes. The
downstream process of enzyme production is enzyme purification. A common method
used in enzyme purification is ultrafiltration membrane technology. However, the use of
ultrafiltration (UF) membrane technology for enzyme purification generally employs
synthetic polymer-based membranes. Synthetic polymers have low degradability, making
them pollutants to the environment. The use of biopolymers is an alternative in enzyme
purification using UF membranes. Bovine Serum Albumin (BSA) is a protein widely used
as a model enzyme in scientific research. This study uses corn prolamin as a biopolymer
for fabricating membranes used for BSA filtration as a model enzyme. The objective of
this research is to determine the influence of the operating conditions in the fabrication
of corn prolamin-based membranes on the formation of membrane structure.
Additionally, this research aims to determine the effect of using corn prolamin-based
membranes on the results of BSA filtration. The variations used are the weight
composition of corn prolamin with solvent (1:9; 2:8; and 3:7), evaporation time (10-20
seconds), and drying time (1-24 hours). Furthermore, BSA filtration was performed using
dead-end filtration with a pressure of 2 bar. The characterization of the morphology of
zein-based membranes conducted includes pore diameter measurement using a Scanning
Electron Microscope (SEM). Based on the research results, it was found that the higher
the concentration of zein used, the lower the porosity level. Longer evaporation times
result in smaller pore sizes of the membrane. Longer drying times make the membrane
stiffer, but the zein coating has better strength. Additionally, the permeate flux from BSA
filtration using zein membranes is greater than that of commercial MF and UF
membranes. The rejection values of the membranes have negative values due to the
collapse of the zein coating, in contrast to commercial MF and UF membranes. |
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