FORMULATION AND CHARACTERIZATION NANOSUSPENSION OF COMBINATION OF MORINGA LEAF EXTRACT (MORINGA OLEIFERA) AND CILEMBU SWEET POTATO (IPOMEA BATATAS) SACCHARIDES AND OXIDATIVE STRESS INHIBITION EFFECT TEST IN VITRO

• Main Author: Samaawaati, Shaafiiana
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/85327
• Institution: Institut Teknologi Bandung

Oxidative stress is a state of imbalance between free radicals and antioxidants, resulting in increased production of Reactive Oxygen Species (ROS) levels that can cause inflammation in the stomach. The addition of external antioxidants is needed to overcome oxidative stress, one of which is by utilizing antioxidant compounds in Moringa oleifera leaves and amino acids contained there in that can improve inflammation. Moringa oleifera leaves will be formulated in the form of freeze-dried nanosuspension to overcome the low bioavailability environment of compounds in Moringa leaves. The purpose of this study was to optimize the homogenization and ultrasonication processes, the concentration of arginine as a stabilizer, and the concentration of cilembu sweet potato saccharides as cryoprotectants. After that, the determination of antioxidant activity in the preparation, viability test on RAW 264.7 cells with or without H2O2 industry, and inhibition test of Nitric Oxide (NO) induced by Lipopolysaccharide (LPS) were carried out. The optimum process was obtained using ultra-turrax at a speed of 5000 rpm, a 5-min bath sonicator, and a 60% probe sonicator for 5 min. The optimum freeze dried nanosuspension formula was obtained with a total extract concentration of 1%, 0.5% arginine, and 10% saccharides which produced a particle size after redispersion of 301.65 ± 46.32 nm. The antioxidant activity of the nanosuspension preparation was obtained with an ascorbic acid equivalent of 95.69 ± 0.85 mg AEAC/g. Viability testing on RAW 264.7 cells, both with and without H2O2, showed that the percentage cell viability of the nanosuspension was better than the single extract and base. The inhibition of NO nanosuspension also showed better results than the single extract and base.