DEVELOPMENT OF ANTIBIOTIC RESISTANT ESCHERICHIA COLI ENUMERATION METHOD BY DETECTING INTRINSIC ?-DGLUCURONIDASE ACTIVITY IN HOSPITAL AND PHARMACEUTICAL INDUSTRY WASTEWATER
Antibiotic resistance has become a significant global health issue, with AntibioticResistant E. coli (AREc) being the most commonly found pathogen. This study analyzes the resistance of E. coli to antibiotics in wastewater from sources of antibiotic residues, namely hospitals and pharmaceutical in...
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| Format: | Final Project |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/86208 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Antibiotic resistance has become a significant global health issue, with AntibioticResistant E. coli (AREc) being the most commonly found pathogen. This study
analyzes the resistance of E. coli to antibiotics in wastewater from sources of
antibiotic residues, namely hospitals and pharmaceutical industries in the Upper
Citarum Watershed. Wastewater samples were taken from the outlets of wastewater
treatment plants (WWTPs) at eleven hospitals and two pharmaceutical industries,
then tested using conventional membrane filter methods and microplate reader
methods with ?-D-Glucuronidase (GUS) activity detection. Each source had
different WWTP efficiencies in removing total coliforms, E. coli, and AREc from the
effluent. The results showed significant variations in the total number of E. coli and
AREc at each sampling point, with some samples exhibiting high resistance to
several antibiotics (multidrug-resistant), particularly amoxicillin, amoxiclav, and
thiamphenicol. Physicochemical parameters such as ammonia, COD, and BOD
were correlated with the amount of AREc detected in the samples. The trend of
AREc resistance was also related to the region's health quality but did not correlate
with the hospital's bed service capacity. The microplate reader method – GUS
activity showed correlation with the conventional membrane filter method at some
WWTP points, demonstrating the potential of this method in detecting AREc, though
further adjustments are needed. This study highlights the need for further research
into the factors and causes of antibiotic resistance, the improvement of wastewater
management and treatment in hospitals and pharmaceutical industries to reduce
the spread of antibiotic resistance, and the encouragement of further development
and broader implementation of ?-D-Glucuronidase activity detection methods for
monitoring AREc in wastewater |
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