#TITLE_ALTERNATIVE#

• Main Author: Sriyati (NIM 206 92 023), Siti
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/9281
• Institution: Institut Teknologi Bandung

Abstract : <br /> <br /> <br /> An investigation on the reproductive biology of Leucaena leucocephala cultivar PG 08 and Leucaena diversifolia cultivar PG 79, was carried out. The purpose of the investigation was to obtain information concerning possible reasons of failure in the pollination process which could be the cause of the small amount of fruit development. The morphological development ofthe inflorescence was followed at the Pondok Gedeh Experimental Station in Bogor. For anatomical observations various developmental stages of the inflorescence were collected and fixed in FAA (formaldehyde 40% : glacial acetic acid : ethyl alcohol 50% = 1:1:18 vv), dehydrated with an ethyl alcohol-tertiary butyl alcohol series, infiltrated and afterwards embedded in paraffine. Serial sections were cut with the rotary microtome and stained with hemalum mayer. The young gynoecium, anther and pollen were fixed in 4% glutaraldehyde and post fixed in osmium tetraoxide. After dehydration with an alcohol series followed by amylacetate the material was dried with the Critical Point Dryer, coated with gold and observed with the Scanning Electron Microscope. Some histochemical methods were used and pollen viability was detected with fluorescein diacetate and also by germination of pollen on an agar medium. Results showed that in both species the long filament developed after the anther, and the long style developed after the ovary and stigma were established. Microsporogenesis formation was finished long before megasporogenesis. Ovule is campylotropous, bitegmic and crassinucellate. The monosporous embryo sac is of the oenothera type. The style is of half-closed type. Factors thought to impede successful pollination in both species were the occurrence of nonhermaphrodite flowers in the inflorescence ; the location of the receptive portion of the stigma within a deep incavation and the occurrence of ovule eating insect larvae. Other factor for Leucaena diversifolia cultivar PG 79 was the low viability of pollen.