Inhibitory activity of Levofloxacin Against MDR Staphylococcus Aureus and Pseudomonas Aeruginosa Clinical Isolates
Staphylococcus aureus and Pseudomonas aeruginosa are the most dangerous and important species among their genus. These bacteria are often resistant to many classes of antimicrobial agents; which make difficulties in selecting appropriate drug to treat infections. Multidrug-resistan...
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| Main Authors: | , , |
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| Format: | Article PeerReviewed |
| Language: | English English |
| Published: |
Fakultas Farmasi Universitas Airlangga
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/102497/1/C-10%20Artkel%20%2B.pdf http://repository.unair.ac.id/102497/2/Tambahan%202%20Validasi%20C-10-signed.pdf http://repository.unair.ac.id/102497/ https://e-journal.unair.ac.id/JFIKI/article/view/13024 http://dx.doi.org/10.20473/jfiki.v6i12019.25-31 |
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| Institution: | Universitas Airlangga |
| Language: | English English |
| Summary: | Staphylococcus aureus and Pseudomonas aeruginosa are the most dangerous and important species among their genus. These bacteria are often resistant to many classes of antimicrobial agents; which make difficulties in selecting appropriate drug to treat infections. Multidrug-resistance occurs readily in hospitals for which antimicrobials agents were used widely.
Objective: The aims of this study was to determine minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of levofloxacin against 22 multidrug resistant- clinical (MDR) strains of Staphylococcus aureus and Pseudomonas aeruginosa isolated from patients pus and urine in hospital.
Methods: Determination of the MIC was performed by macro-dilution broth assay as recommended by Clinical and Laboratory Standards Institute (CLSI), while the MBC was determined one-step further after the MIC determination.
Results: It was found that MIC of the levofloxacin were (0.3 ± 0.0) - >0.5 µg/mL and (0.2 ± 0.1) - (1.0 ±0.0)µg/mL against S. aureus from pus and urine, respectively. In addition, higher MICs were yielded against P. aeruginosa, (1.0 ± 0.0) - >8.0 µg/mL and (0.7 ± 0.3) - (3.0 ± 1.2) µg/mL for pus and urine isolates respectively. Similar to MICs, the MBCs against P. aeruginosa were higher than S. aureus, (0.6 ± 0.0) - > 4.0 µg/mL and (0.3 ± 0.0) - >8.0 µg/mL isolated from pus and urine respectively, (2.0 ± 0.6) - > 8.0 µg/mL and (3.0 ± 1.2) - >7.0 µg/mL against P. aeruginosa from pus and urine respectively.
Conclusion: The levofloxacin was still susceptible as bacteriostatic against isolates from both body fluids, but not bactericidal towards all isolates. |
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