Inhibitory activity of Levofloxacin Against MDR Staphylococcus Aureus and Pseudomonas Aeruginosa Clinical Isolates

• Main Authors: Lisa Nathalie, Lindawati Alimsardjono, Isnaeni
• Format: Article PeerReviewed
• Language: English; English
• Published: Fakultas Farmasi Universitas Airlangga 2019
• Subjects: R Medicine; RS Pharmacy and materia medica
• Online Access: http://repository.unair.ac.id/102497/1/C-10%20Artkel%20%2B.pdf; http://repository.unair.ac.id/102497/2/Tambahan%202%20Validasi%20C-10-signed.pdf; http://repository.unair.ac.id/102497/; https://e-journal.unair.ac.id/JFIKI/article/view/13024; http://dx.doi.org/10.20473/jfiki.v6i12019.25-31
• Institution: Universitas Airlangga

Staphylococcus aureus and Pseudomonas aeruginosa are the most dangerous and important species among their genus. These bacteria are often resistant to many classes of antimicrobial agents; which make difficulties in selecting appropriate drug to treat infections. Multidrug-resistance occurs readily in hospitals for which antimicrobials agents were used widely. Objective: The aims of this study was to determine minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of levofloxacin against 22 multidrug resistant- clinical (MDR) strains of Staphylococcus aureus and Pseudomonas aeruginosa isolated from patients pus and urine in hospital. Methods: Determination of the MIC was performed by macro-dilution broth assay as recommended by Clinical and Laboratory Standards Institute (CLSI), while the MBC was determined one-step further after the MIC determination. Results: It was found that MIC of the levofloxacin were (0.3 ± 0.0) - >0.5 µg/mL and (0.2 ± 0.1) - (1.0 ±0.0)µg/mL against S. aureus from pus and urine, respectively. In addition, higher MICs were yielded against P. aeruginosa, (1.0 ± 0.0) - >8.0 µg/mL and (0.7 ± 0.3) - (3.0 ± 1.2) µg/mL for pus and urine isolates respectively. Similar to MICs, the MBCs against P. aeruginosa were higher than S. aureus, (0.6 ± 0.0) - > 4.0 µg/mL and (0.3 ± 0.0) - >8.0 µg/mL isolated from pus and urine respectively, (2.0 ± 0.6) - > 8.0 µg/mL and (3.0 ± 1.2) - >7.0 µg/mL against P. aeruginosa from pus and urine respectively. Conclusion: The levofloxacin was still susceptible as bacteriostatic against isolates from both body fluids, but not bactericidal towards all isolates.