Development of Spore Protein of Myxobolus koi as an Immunostimulant for Prevent of Myxobolusis on Gold Fish (Cyprinus carpio Linn) by Oral Immunisation
Production of Gold fish (Cyprinus carpio Linn) in Indonesia has always increased from 2013 to 2015 year by year with increasing average 2% per year. The amount of production was respectively 571.892 tonnes, 1129.273 tonnes, and 1186.674 tonnes. There were almost no problems to sale of gold fish...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article PeerReviewed |
| Language: | English English Indonesian English Indonesian Indonesian |
| Published: |
IOP Conference Series: Earth and Environmental Science
2017
|
| Subjects: | |
| Online Access: | https://repository.unair.ac.id/113497/1/J1.67%20IOP.pdf https://repository.unair.ac.id/113497/4/C67.pdf https://repository.unair.ac.id/113497/6/C.67_Baru.pdf https://repository.unair.ac.id/113497/7/67.pdf https://repository.unair.ac.id/113497/8/67-1.pdf https://repository.unair.ac.id/113497/10/57-Development%20of%20Spore-1.pdf https://repository.unair.ac.id/113497/ https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012009/pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universitas Airlangga |
| Language: | English English Indonesian English Indonesian Indonesian |
| Summary: | Production of Gold fish (Cyprinus carpio Linn) in Indonesia has always
increased from 2013 to 2015 year by year with increasing average 2% per year. The amount
of production was respectively 571.892 tonnes, 1129.273 tonnes, and 1186.674 tonnes.
There were almost no problems to sale of gold fish because it had a good enough prospect.
The aims of this research were Isolation of spore protein of Myxobolus koi by using SDS�PAGE to analyze immun respons and survival rate gold fish that immunized with spore
protein of Myxobolus koi. The method of this research used experimental method, and
belonged to 4 treatments that are: Control, the group of gold fish not immunized with
protein spore of Myxobolus koi neither infected by Myxobolus koi (T1). The group
immunized and infested by spore of Myxobolus koi (T2). The group which immunized and
not infested by Myxobolus koi (T3), and The group only infested by Myxobolus koi (T4).
The dose of immunostimulant was 5 ml in 1 kg of food. The result showed that there were
two bands of whole spore protein with molecule weight (MW) 150 kDa and 72 kDa and
one band of crude protein Myxobolus koi with molecule weight 73 kD and the optical
density point was 0.132 on the first day and increased to 0.769 on the 56 th day. The result
also showed that the immun respons and survival rate increased from 27% to 86% in
chellence test. The protein spore of Myxobolus koi can used to develop material for
immunostimulant and to prevent the myxobolusis. |
|---|