The squamous cell carcinoma cell line OM-1 retains both p75-dependent stratified epithelial progenitor potential and cancer stem cell properties

• Main Authors: Ryo Uetsuki, -, Koichiro Higashikawa, -, Satoshi Okuda, -, Nao Yamakado, -, Fumi Ishida, -, Andra Rizqiawan, -, Shigehiro Ono, -, Masaaki Takechi, -, Kuniko Mizuta, -, Hideo Shigeishi, -, Nobuyuki Kamata, -, Kei Tobiume, -
• Format: Article PeerReviewed
• Language: English; English; Indonesian; English
• Published: 2021
• Subjects: R Medicine; RK Dentistry
• Online Access: https://repository.unair.ac.id/116759/1/12.The%20squamous%20cell%20carcinoma.pdf; https://repository.unair.ac.id/116759/2/10.%20tiurnitin%20The%20squamous%20cell%20carcinoma%20cell%20line.pdf; https://repository.unair.ac.id/116759/3/Artikel%2012%20Andra%20Rizqiawan.pdf; https://repository.unair.ac.id/116759/6/Karil%20%2019%20Prof%20Indri%20SCC%20OM1.pdf; https://repository.unair.ac.id/116759/; https://doi.org/10.1016/j.bbrep.2021.101003
• Institution: Universitas Airlangga

The low-affinity nerve growth factor receptor p75 is a stratified epithelial stem/progenitor marker of human epithelia. We found OM-1, a human squamous cell carcinoma (SCC) cell line, showed distinct cells with p75 cluster, especially located at the center of a growing colony in a monolayer culture. A cell with p75 cluster was surrounded by cytokeratin 14- and cytokeratin 13-expressing cells that settled at the outer margin of the colony. OM-1 cells were also capable of forming tumor spheres in a cell suspension culture, an ability which was attenuated by the inhibition of p75-signaling. Intriguingly, we also found a p75-negative cell population from a growing culture of OM-1 that re-committed to become p75-clustering cells. These results indicated the possibility that SCC with epithelial multi-layering capacity can exploit the p75-dependent stratified epithelial progenitor property for the cancer stemness.