Citrus limon Peel Essential Oil–Induced Type IV Hypersensitivity Reaction
Background: Several studies have shown anti-inflammatory, anti-microbial, antifungal, and antioxidant effects from Citrus limon–peel essential oil (Cl-PEO). Cl-PEO can be developed as topical drugs for oral ulceration because of its potential active components. There have been no studies on the t...
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| Main Authors: | , , , , , , , , |
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| Format: | Article PeerReviewed |
| Language: | English English English |
| Published: |
Dove press
2020
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/116826/1/10.Citrus%20limon%20peel%20essential%20oil%20induced%20type%20IV%20hypersensitivity%20reaction_compressed.pdf https://repository.unair.ac.id/116826/2/10.Citrus%20limon%20Peel%20Essential%20Oil%E2%80%93Induced%20Type%20IV%20Hypersensitivity%20Reaction%282%29_compressed.pdf https://repository.unair.ac.id/116826/5/10.%20Citrus%20Limon%20L%20Peel%20essential_compressed.pdf https://repository.unair.ac.id/116826/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7394596/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Background: Several studies have shown anti-inflammatory, anti-microbial, antifungal, and
antioxidant effects from Citrus limon–peel essential oil (Cl-PEO). Cl-PEO can be developed
as topical drugs for oral ulceration because of its potential active components. There have
been no studies on the topical application of Cl-PEO inducing type IV hypersensitivity
reaction.
Purpose: To investigate the potential of Cl-PEO from Batu City to induce type IV
hypersensitivity reactions based on clinical changes, lymphocytes, macrophages, IFNγ,
andIL10 expression.
Methods: This study was adapted from a guinea pig maximization-test method in Indonesia
and the guidance of ISO 10,993-10:2010, and conducted on 20 guinea pigs (Cavia cobaya)
divided into a control group and a treatment group. The treatment group was given Cl-PEO
and the control group CMC-Na. Clinical changes were observed, then tissue specimens taken
for hematoxylin–eosin and immunohistochemistry staining.
Results: There were no clinical changes after exposure. Lymphocyte and macrophage
numbers and IFNγ and IL10 expression increased in the treatment group compared to the
control group (p=0).
Conclusion: Cl-PEO can induce type IV hypersensitivity reactions in guinea pigs based on
cellular and molecular cytokines, but there are no clinical changes after topical application. |
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