Correlation of HIF-1α, CXCR4 and MMP13 Expression in Laryngeal Squamous Cell Carcinoma with Cervical Nodal Status

• Main Authors: Etty Hary Kusumastuti, Etty, Anny Setijo Rahaju, Anny, Alphania Rahniayu, Alphania, Sjahjenny Mustokoweni, Sjahjenny, Rovi Anggoro, Rovi, Muhtarum Yusuf, Muhtarum, I Ketut Sudiana, I Ketut
• Format: Article PeerReviewed
• Language: English; Indonesian; English; Indonesian; Indonesian
• Published: Medico Legal Society
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/122579/1/20%20ARTIKEL.pdf; https://repository.unair.ac.id/122579/2/20%20etik.pdf; https://repository.unair.ac.id/122579/4/20%20turnitin.pdf; https://repository.unair.ac.id/122579/9/Karil%20%281%29-20.pdf; https://repository.unair.ac.id/122579/11/19%20karil.pdf; https://repository.unair.ac.id/122579/; https://medicopublication.com/index.php/ijfmt/article/view/18426; https://doi.org/10.37506/ijfmt.v15i4.18426
• Institution: Universitas Airlangga

Abstract Background: Laryngeal carcinoma is the most common malignancy of the upper respiratory tract, more than 98% are squamous cell carcinoma (SCC). New method is necessary for identifying and αpredicting nodal metastasis in laryngeal SCC. Overproliferating tumor cells will induce hypoxia and release HIF-1α, which in turn will upregulate CXCR4. CXCR4 then will induce MMP13, a protein that degrade extracellular matrix (ECM), thus promoting metastatic process. Methods: A cross sectional study, using 30 samples of laryngeal SCC, divided into two groups: based on cervical lymph node status. All samples were stained immunohistochemically against HIF-1α, CXCR4 and MMP13 antibody. The expressions were evaluated using immunoreactive score (IRS). Result: There were significant difference among HIF1α, CXCR4, and MMP13 in laryngeal SCC with positive nodal metastasis group compared to negative nodal metastasis group (p<0,05). Conclusion: There were significant differences in HIF-1α, CXCR4 and MMP13 immunoexpression in laryngeal SCC with cervical nodal metastasis compared to laryngeal SCC without cervical nodal metastasis. There was a positive correlation between HIF-1α and MMP13 expression, positive correlation between CXCR4 and MMP13 expression, but there was no correlation between HIF-1α and CXCR4 expression.