Chitosan Antibacterial Activity Against Streptococcus viridans
Introduction: Streptococcus viridans is the most common facultative anaerobic bacteria which can cause pulp and root canal infections in oral cavity. This Gram-positive bacterium can cause pulp necrosis, an indication for root canal treatment. One of the important steps in root canal treatment is...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Article PeerReviewed |
Language: | English English English |
Published: |
2021
|
Subjects: | |
Online Access: | https://repository.unair.ac.id/123616/1/7.pdf https://repository.unair.ac.id/123616/2/7.pdf https://repository.unair.ac.id/123616/3/7.pdf https://repository.unair.ac.id/123616/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universitas Airlangga |
Language: | English English English |
Summary: | Introduction: Streptococcus viridans is the most common facultative anaerobic bacteria which can
cause pulp and root canal infections in oral cavity. This Gram-positive bacterium can cause pulp
necrosis, an indication for root canal treatment. One of the important steps in root canal treatment
is root canal irrigation and a good root canal irrigant has a maximum antibacterial effect with
minimum toxicity effect. Chitosan is a natural ingredient from shrimp shells. Chitosan has high
antibacterial activity against many pathogenic microorganisms and has been used as an antibacterial agent
to eliminate Streptococcus viridans. Methods: Streptococcus viridans was grown in a tube which
contained BHIB medium and 2% chitosan suspension in each sample group with the concentration of
0.0156%; 0.03125%; .0625%; 0.125%; 0.25%; 0.5%; 1%; 2%. Positive control (BHIB medium and
Streptococcus viridans bacteria), 2% acetic acid solution control (2% acetic acid solution and Streptococcus
viridans), and negative control (BHIB medium) were made to ensure that there was not any antibacterial
effect on the medium. Each tube was incubated at 37°C for 24 hours. The number of living colonies were
calculated and compared with the positive control to determine the antibacterial activity, MIC, and MBC.
Results: In this study, the percentage of the living colonies in the sample groups with chitosan
concentration of 0.125%; 0.0625%; 0.03125% were 0%; 7.2%; 16.4%, respectively. Conclusion: Chitosan
has an antibacterial activity with 0.0625% MIC and 0.0125% MBC against Streptococcus viridans. |
---|