Chitosan Antibacterial Activity Against Streptococcus viridans
Introduction: Streptococcus viridans is the most common facultative anaerobic bacteria which can cause pulp and root canal infections in oral cavity. This Gram-positive bacterium can cause pulp necrosis, an indication for root canal treatment. One of the important steps in root canal treatment is...
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id-langga.1236162023-04-17T00:26:53Z https://repository.unair.ac.id/123616/ Chitosan Antibacterial Activity Against Streptococcus viridans Dian Agustin Wahjuningrum, - Halida Dwi Pramesti, - Manuel Raynaldy Sihombing, - Zabrina Luthfiana Devi, - M. Roelianto, - Setyabudi, - RK Dentistry Introduction: Streptococcus viridans is the most common facultative anaerobic bacteria which can cause pulp and root canal infections in oral cavity. This Gram-positive bacterium can cause pulp necrosis, an indication for root canal treatment. One of the important steps in root canal treatment is root canal irrigation and a good root canal irrigant has a maximum antibacterial effect with minimum toxicity effect. Chitosan is a natural ingredient from shrimp shells. Chitosan has high antibacterial activity against many pathogenic microorganisms and has been used as an antibacterial agent to eliminate Streptococcus viridans. Methods: Streptococcus viridans was grown in a tube which contained BHIB medium and 2% chitosan suspension in each sample group with the concentration of 0.0156%; 0.03125%; .0625%; 0.125%; 0.25%; 0.5%; 1%; 2%. Positive control (BHIB medium and Streptococcus viridans bacteria), 2% acetic acid solution control (2% acetic acid solution and Streptococcus viridans), and negative control (BHIB medium) were made to ensure that there was not any antibacterial effect on the medium. Each tube was incubated at 37°C for 24 hours. The number of living colonies were calculated and compared with the positive control to determine the antibacterial activity, MIC, and MBC. Results: In this study, the percentage of the living colonies in the sample groups with chitosan concentration of 0.125%; 0.0625%; 0.03125% were 0%; 7.2%; 16.4%, respectively. Conclusion: Chitosan has an antibacterial activity with 0.0625% MIC and 0.0125% MBC against Streptococcus viridans. 2021 Article PeerReviewed text en https://repository.unair.ac.id/123616/1/7.pdf text en https://repository.unair.ac.id/123616/2/7.pdf text en https://repository.unair.ac.id/123616/3/7.pdf Dian Agustin Wahjuningrum, - and Halida Dwi Pramesti, - and Manuel Raynaldy Sihombing, - and Zabrina Luthfiana Devi, - and M. Roelianto, - and Setyabudi, - (2021) Chitosan Antibacterial Activity Against Streptococcus viridans. Malaysian Journal of Medicine and Health Sciences. |
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RK Dentistry Dian Agustin Wahjuningrum, - Halida Dwi Pramesti, - Manuel Raynaldy Sihombing, - Zabrina Luthfiana Devi, - M. Roelianto, - Setyabudi, - Chitosan Antibacterial Activity Against Streptococcus viridans |
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Introduction: Streptococcus viridans is the most common facultative anaerobic bacteria which can
cause pulp and root canal infections in oral cavity. This Gram-positive bacterium can cause pulp
necrosis, an indication for root canal treatment. One of the important steps in root canal treatment
is root canal irrigation and a good root canal irrigant has a maximum antibacterial effect with
minimum toxicity effect. Chitosan is a natural ingredient from shrimp shells. Chitosan has high
antibacterial activity against many pathogenic microorganisms and has been used as an antibacterial agent
to eliminate Streptococcus viridans. Methods: Streptococcus viridans was grown in a tube which
contained BHIB medium and 2% chitosan suspension in each sample group with the concentration of
0.0156%; 0.03125%; .0625%; 0.125%; 0.25%; 0.5%; 1%; 2%. Positive control (BHIB medium and
Streptococcus viridans bacteria), 2% acetic acid solution control (2% acetic acid solution and Streptococcus
viridans), and negative control (BHIB medium) were made to ensure that there was not any antibacterial
effect on the medium. Each tube was incubated at 37°C for 24 hours. The number of living colonies were
calculated and compared with the positive control to determine the antibacterial activity, MIC, and MBC.
Results: In this study, the percentage of the living colonies in the sample groups with chitosan
concentration of 0.125%; 0.0625%; 0.03125% were 0%; 7.2%; 16.4%, respectively. Conclusion: Chitosan
has an antibacterial activity with 0.0625% MIC and 0.0125% MBC against Streptococcus viridans. |
format |
Article PeerReviewed |
author |
Dian Agustin Wahjuningrum, - Halida Dwi Pramesti, - Manuel Raynaldy Sihombing, - Zabrina Luthfiana Devi, - M. Roelianto, - Setyabudi, - |
author_facet |
Dian Agustin Wahjuningrum, - Halida Dwi Pramesti, - Manuel Raynaldy Sihombing, - Zabrina Luthfiana Devi, - M. Roelianto, - Setyabudi, - |
author_sort |
Dian Agustin Wahjuningrum, - |
title |
Chitosan Antibacterial Activity Against Streptococcus viridans |
title_short |
Chitosan Antibacterial Activity Against Streptococcus viridans |
title_full |
Chitosan Antibacterial Activity Against Streptococcus viridans |
title_fullStr |
Chitosan Antibacterial Activity Against Streptococcus viridans |
title_full_unstemmed |
Chitosan Antibacterial Activity Against Streptococcus viridans |
title_sort |
chitosan antibacterial activity against streptococcus viridans |
publishDate |
2021 |
url |
https://repository.unair.ac.id/123616/1/7.pdf https://repository.unair.ac.id/123616/2/7.pdf https://repository.unair.ac.id/123616/3/7.pdf https://repository.unair.ac.id/123616/ |
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