Effect of Glutamine on Apoptosis-inducing Factor Expression and Apoptosis of Glomerular Parietal Epithelial Cells of Cisplatin-exposed Rats

• Main Authors: Ihsan Fahmi Rofananda, Ihsan, Jusak Nugraha, Jusak, Imam Susilo, Imam, Miyayu Soneta Sofyan, Miyayu
• Format: Article PeerReviewed
• Language: English; English; Indonesian; English; Indonesian
• Published: Scientific Foundation SPIROSKI
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/124174/1/05.%20artikel.pdf; https://repository.unair.ac.id/124174/2/05.%20Effect%20of%20Glutamine%20on%20Apoptosis-inducing.pdf; https://repository.unair.ac.id/124174/3/5karil.pdf; https://repository.unair.ac.id/124174/4/05.%20Turnitin.pdf; https://repository.unair.ac.id/124174/9/05.%20etik.pdf; https://repository.unair.ac.id/124174/; https://oamjms.eu/index.php/mjms/article/view/5915; https://doi.org/10.3889/oamjms.2021.5915
• Institution: Universitas Airlangga

Abstract AIM: This study analyzed the nephroprotective effect by examining apoptosis-inducing factor (AIF) expression and apoptosis rate in the glomerular parietal epithelial cell of cisplatin-exposed rats. METHODS: Samples consisted of 30 rats (divided into 3 groups: Group P0 received no treatment, group P1 received a cisplatin injection on the 7th day, and group P2 received glutamine injection on days 1–7 and cisplatin injection on the 7th day). After 72 h, the tissue samples were immunohistochemically processed. AIF expression was measured in an Allred score. The apoptosis rate was measured in apoptotic cells/field of view. Statistical analysis was carried out using JASP Statistics ver. 0.12.0 (p < 0.05). RESULTS: AIF expression values are follows: P0 = 4.89 ± 0.418, P1 = 6.14 ± 0.685, and P2 = 4.95 ± 0.530. The Kruskal–Wallis test result showed a significant difference (p < 0.05) between the groups and Dunn’s post hoc test showed a significant difference between P0 and P1 and between P1 and P2, but no significant difference between P0 and P2. Meanwhile, apoptosis rate values are as follows: P0 = 24.3 ± 9.821, P1 = 123.6 ± 16.008, and P2 = 77.2 ± 10.644. The Kruskal–Wallis test result showed a significant difference (p < 0.05) between the groups, and Dunn’s post hoc test showed a significant difference between P0 and P1, between P1 and P2, and between P0 and P2. CONCLUSION: The expression of AIF and apoptosis of glomerular parietal epithelial cells of the cisplatin-exposed rat has decreased after glutamine treatment.