The correlation between vitamin D and levels of IFN-γ, NF-κB, thyroid antibodies in down syndrome: study in Indonesian children

• Main Authors: Yuni Hisbiyah, -, Anang Endaryanto, -, Bagus Setyoboedi, Bagus, Nur Rochmah, -, Muhammad Faizi, -
• Format: Article PeerReviewed
• Language: English; Indonesian; English; Indonesian
• Published: Mattioli1885
• Subjects: R5-920 Medicine (General)
• Online Access: https://repository.unair.ac.id/127769/1/07.%20The%20correlation%20between.pdf; https://repository.unair.ac.id/127769/2/07%20karil.pdf; https://repository.unair.ac.id/127769/3/07.%20The%20correlation%20between%20vitamin.pdf; https://repository.unair.ac.id/127769/7/7%20etik.pdf; https://repository.unair.ac.id/127769/; https://mattioli1885journals.com/index.php/actabiomedica/article/view/13722; https://doi.org/10.23750/abm.v93i6.13722
• Institution: Universitas Airlangga

Abstract Background and aim: Vitamin D (VD) reduces interferon-gamma (IFN-γ) production and prevents nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, impacting the inhibition of the autoimmunity process such as autoimmune thyroiditis (AITD). Children with Down syndrome (DS) are reported to have a higher risk of autoimmunity and lower VD levels than non-DS. Therefore, this study aimed to evaluate VD levels in Indonesian DS children and their relationship with marker of AITD. Methods: This study was conducted on DS children at Dr Soetomo Hospital between February 2021-June 2022. Socio-demographic status, amount of milk, fish and meat consumption, and duration of sun exposure were obtained using a self-report questionnaire. Thyroid hormone (TSH and FT4), thyroid antibody (TPO-Ab and Tg-Ab), 25 (OH)D, IFN-γ, and NF-κB levels were measured using ELISA. Results: Of the 80 participants, 53.75% had sufficient (50.829±17.713 ng/ml) and 46.25% had non-sufficient (20.606±5.974 ng/ml) VD levels. Daily milk consumption, meat and fish consumption were risk factors contributing to VD levels in multivariate analysis [p=0.003, OR=1.007(1.003–1.012); p=0.004, OR=1.816(1.209– 2.728), respectively]. Participants with sufficient VD had significantly higher TPO-Ab (p=0.007) and Tg-Ab (p=0.016). Mean of VD levels were significantly negatively correlated with IFN-γ levels (r =-0.262, p=0.037) and positively correlated with TPO-Ab (r= 0.432, p=1x10-5,) and Tg-Ab (r= 0.375, p=0.001). Conclusions: Majority of subjects had sufficient VD levels. VD suppresses IFN-g, but is unable to affect NF-κB levels, presumably causing high levels of TPO-Ab and Tg-Ab in sufficient VD patient.