Analisis Imunogenisitas Virus Dengue Inaktif (Denv-I, Denv-2, Denv-3, Derv-4) pada Mencit (Mus Musculus) sebagai Kandidat Vaksin Koktail Dengue
Dengue virus (DENV) is a single stranded RNA virus that circulates in the body of primate and causes DHF for human. Dengue virus have four serotypes (DENV-l, DENV-2, DENV-3, DENV-4). Four dengue virus serotypes do have a similar, yet distinct antigenic properties. Infection only one serotype will...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/129873/1/KKC%20KK%20TKH%20Dek%20a.pdf https://repository.unair.ac.id/129873/ http://lib.unair.ac.id/ |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian |
| Summary: | Dengue virus (DENV) is a single stranded RNA virus that circulates in the body
of primate and causes DHF for human. Dengue virus have four serotypes
(DENV-l, DENV-2, DENV-3, DENV-4). Four dengue virus serotypes do have a
similar, yet distinct antigenic properties. Infection only one serotype will give
immunity to one serotype is concerned, but does not provide cross- immunity.
This study aims to determine the immunogenicity of dengue virus (DENV-l,
DENV-2, DENV-3 and DENV-4) inactive as a candidate dengue cocktail vaccine.
This study used 18 mices were divided into three treatment groups, PO immunized
0.5 cc PBS, PI immunized with cocktail of dengue virus (DENV-l, DENV-2,
DENV-3 and DENV-4) inactive as much as 0.5 cc TCIDsol07
, P2 immunized
cocktail of dengue virus (DENV-l, DENV-2, DENV-3 and DENV-4) inactive as
much as 0.5 cc TCIDso108. At day 0,7,14 and 21 days of 18 mice that had been
treated blood drawn to determine the levels of immunoglobulin M and G with
indirect elisa method. On day 28, the treatment group PI and P2 in the challenge
test with a cocktail immunized with dengue virus (DENV-l, DENV-2, DENV-3
and DENV -4) inactive 0.5 cc TCIDso108 for the presence of viremia. The results
of this study are significant differences (p<0.05) between the groups PO (control),
PI and P2 in terms of inducing titers of immunoglobulin (Ig M, Ig G, Ig GIA, Ig
G2a and Ig G2B ) . In the challenge test using inactivated dengue virus (DENV-l,
DENV-2, DENV-3 and DENV-4) TCIDsol08 in experimental animals (PI and
P2), then the PCR test followed by electrophoresis results showed the absence of
viremia or viral dengue as evidenced by a negative PCR result. |
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