UJI AKTIVITAS ANTIMIKROBA EKSTRAK BONGGOL NANAS TERHADAP Methicillin-Resistant Staphylococcus aureus (MRSA)
Staphylococcus aureus is one of major pathogens causing serious infection. Antibiotic is main therapy of Staphylococcus infection. However, inadequate and irrational use of antibiotic causes resistance against penicillin and cephalosporin antibiotics and emerges incidence of methicillin-resistant...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/53259/2/FK.PD.60-16%20Put%20u%20Abstrak.pdf http://repository.unair.ac.id/53259/13/FK.PD.60-16%20Put%20u.pdf http://repository.unair.ac.id/53259/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | Staphylococcus aureus is one of major pathogens causing serious infection.
Antibiotic is main therapy of Staphylococcus infection. However, inadequate and
irrational use of antibiotic causes resistance against penicillin and cephalosporin
antibiotics and emerges incidence of methicillin-resistant Staphylococcus aureus
(MRSA). Use of herbal medicine from pineapple core extract is hopefully can reduce
the incidence of antibiotics resistance. This research was conducted to investigate the
antimicrobial activity of pineapple core extract against methicillin-resistant
Staphylococcus aureus (MRSA).
This research is true experimental with post-test controlled group design that
was done in microbiology laboratory. Pineapple core was extracted by maceration
method. Ethanol extract of pineapple core is dissolved with sterilized water. The
minimum inhibitory concentration (MIC) and minimum bactericidal concentration
(MBC) were determined by broth dilution test. Concentrations of extract used in the
research were 1500, 1000, 500, 375, 250, and 125 mg/ml. Vancomycin antibiotic was
used as control group. The replication used in the research was five replications. MIC
was observed visually by comparing turbidity of solutions after incubation at 37oC for
24 hours. Then these solutions were cultured on nutrient agar plates at 37oC for 24
hours. MBC was observed visually by inspecting the presence of bacterial colonies
growth.
The Minimum Inhibitory Concentration (MIC) could not be determined due to
no turbidity changes. Cultures on nutrient agar plates had no colonies growth in
concentrations of 1500 and 1000 mg/ml. Thus, the Minimum Bactericidal
Concentration (MBC) was 1000 mg/ml.
Pineapple core extract contains bromelain, flavonoid, saponin, and tanin, which
are based on studies shown antimicrobial effect. Pineapple has been experimentally
used for antimicrobial susceptibility testing against both gram positive and gram
negative bacteria and shows any antimicrobial effects. In summary, pineapple core
extract has antimicrobial effect against methicillin-resistant Staphylococcus aureus
(MRSA) with MBC of 1000 mg/ml.
Keywords: pineapple core, antimicrobial activity, dilution susceptibility test,
Methicillin-resistant Staphylococcus aureus (MRSA). |
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