Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai
The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL a...
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Departemen Reproduksi Fakultas Kedokteran Hewan
2014
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id-langga.592672017-07-20T18:13:05Z http://repository.unair.ac.id/59267/ Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai Novia Candrawati Suherni Susilowati, NIDN. 0026065905 Bambang Poernomo S. Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology SF811-909 Veterinary medicine of special organs, regions, and systems The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL assay metode using Apo-BrdU-IHCTM In Situ DNA Fragmentation Assay Kit. The result of evaluation of nuclear DNA damage showed significant difference (p<0,05), it was perhaps caused by the composition of soybean lecithin diluter was more complete with the present of antioxidant. The result of spermatozoan quality measurement showed there were no significant difference except for the motility that showed significant difference before sentrifugation (p<0,05). Departemen Reproduksi Fakultas Kedokteran Hewan 2014-10 Article PeerReviewed text en http://repository.unair.ac.id/59267/1/karil%2024%20%28pengukuran%20kerusakan%20dna%20inti%29.pdf text id http://repository.unair.ac.id/59267/2/peerreview%20karil%2024%20%28pengukuran%20kerusakan%29.pdf Novia Candrawati and Suherni Susilowati, NIDN. 0026065905 and Bambang Poernomo S. (2014) Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai. Ovozoa E-Journal, 3 (2). pp. 225-230. ISSN 2302-6464 http://journal.unair.ac.id/measurement-of-nuclear-dna-damage-of-simental-bull-spermatozoa-post-thawing-with-sentrifuged-egg-yolk-skim-and-soybean-lecitin-diluter-article-7966-media-123-category-5.html |
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Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology SF811-909 Veterinary medicine of special organs, regions, and systems |
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Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology SF811-909 Veterinary medicine of special organs, regions, and systems Novia Candrawati Suherni Susilowati, NIDN. 0026065905 Bambang Poernomo S. Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai |
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The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL assay metode using Apo-BrdU-IHCTM In Situ DNA Fragmentation Assay Kit. The result of evaluation of nuclear DNA damage showed significant difference (p<0,05), it was perhaps caused by the composition of soybean lecithin diluter was more complete with the present of antioxidant. The result of spermatozoan quality measurement showed there were no significant difference except for the motility that showed significant difference before sentrifugation (p<0,05). |
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Article PeerReviewed |
author |
Novia Candrawati Suherni Susilowati, NIDN. 0026065905 Bambang Poernomo S. |
author_facet |
Novia Candrawati Suherni Susilowati, NIDN. 0026065905 Bambang Poernomo S. |
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Novia Candrawati |
title |
Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai |
title_short |
Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai |
title_full |
Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai |
title_fullStr |
Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai |
title_full_unstemmed |
Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai |
title_sort |
measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = pengukuran kerusakan dna inti spermatozoa sapi simental post thawing yang disentrifugasi menggunakan diluter skim kuning telur dan lesitin kacang kedelai |
publisher |
Departemen Reproduksi Fakultas Kedokteran Hewan |
publishDate |
2014 |
url |
http://repository.unair.ac.id/59267/1/karil%2024%20%28pengukuran%20kerusakan%20dna%20inti%29.pdf http://repository.unair.ac.id/59267/2/peerreview%20karil%2024%20%28pengukuran%20kerusakan%29.pdf http://repository.unair.ac.id/59267/ http://journal.unair.ac.id/measurement-of-nuclear-dna-damage-of-simental-bull-spermatozoa-post-thawing-with-sentrifuged-egg-yolk-skim-and-soybean-lecitin-diluter-article-7966-media-123-category-5.html |
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