Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai

The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL a...

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Main Authors: Novia Candrawati, Suherni Susilowati, NIDN. 0026065905, Bambang Poernomo S.
Format: Article PeerReviewed
Language:English
Indonesian
Published: Departemen Reproduksi Fakultas Kedokteran Hewan 2014
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Online Access:http://repository.unair.ac.id/59267/1/karil%2024%20%28pengukuran%20kerusakan%20dna%20inti%29.pdf
http://repository.unair.ac.id/59267/2/peerreview%20karil%2024%20%28pengukuran%20kerusakan%29.pdf
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http://journal.unair.ac.id/measurement-of-nuclear-dna-damage-of-simental-bull-spermatozoa-post-thawing-with-sentrifuged-egg-yolk-skim-and-soybean-lecitin-diluter-article-7966-media-123-category-5.html
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spelling id-langga.592672017-07-20T18:13:05Z http://repository.unair.ac.id/59267/ Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai Novia Candrawati Suherni Susilowati, NIDN. 0026065905 Bambang Poernomo S. Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology SF811-909 Veterinary medicine of special organs, regions, and systems The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL assay metode using Apo-BrdU-IHCTM In Situ DNA Fragmentation Assay Kit. The result of evaluation of nuclear DNA damage showed significant difference (p<0,05), it was perhaps caused by the composition of soybean lecithin diluter was more complete with the present of antioxidant. The result of spermatozoan quality measurement showed there were no significant difference except for the motility that showed significant difference before sentrifugation (p<0,05). Departemen Reproduksi Fakultas Kedokteran Hewan 2014-10 Article PeerReviewed text en http://repository.unair.ac.id/59267/1/karil%2024%20%28pengukuran%20kerusakan%20dna%20inti%29.pdf text id http://repository.unair.ac.id/59267/2/peerreview%20karil%2024%20%28pengukuran%20kerusakan%29.pdf Novia Candrawati and Suherni Susilowati, NIDN. 0026065905 and Bambang Poernomo S. (2014) Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai. Ovozoa E-Journal, 3 (2). pp. 225-230. ISSN 2302-6464 http://journal.unair.ac.id/measurement-of-nuclear-dna-damage-of-simental-bull-spermatozoa-post-thawing-with-sentrifuged-egg-yolk-skim-and-soybean-lecitin-diluter-article-7966-media-123-category-5.html
institution Universitas Airlangga
building Universitas Airlangga Library
country Indonesia
collection UNAIR Repository
language English
Indonesian
topic Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
SF811-909 Veterinary medicine of special organs, regions, and systems
spellingShingle Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
SF811-909 Veterinary medicine of special organs, regions, and systems
Novia Candrawati
Suherni Susilowati, NIDN. 0026065905
Bambang Poernomo S.
Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai
description The research was aimed to compare egg yolk skim and soybean lecithin diluter after sentrifugation. Comparison both diluter measured by nuclear DNA damage of Simental bull spermatozoa. All parameter were measured before and after sentrifugation. Apoptotic sperm nuclear damage was measured by TUNNEL assay metode using Apo-BrdU-IHCTM In Situ DNA Fragmentation Assay Kit. The result of evaluation of nuclear DNA damage showed significant difference (p<0,05), it was perhaps caused by the composition of soybean lecithin diluter was more complete with the present of antioxidant. The result of spermatozoan quality measurement showed there were no significant difference except for the motility that showed significant difference before sentrifugation (p<0,05).
format Article
PeerReviewed
author Novia Candrawati
Suherni Susilowati, NIDN. 0026065905
Bambang Poernomo S.
author_facet Novia Candrawati
Suherni Susilowati, NIDN. 0026065905
Bambang Poernomo S.
author_sort Novia Candrawati
title Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai
title_short Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai
title_full Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai
title_fullStr Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai
title_full_unstemmed Measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = Pengukuran Kerusakan Dna Inti Spermatozoa Sapi Simental Post Thawing Yang Disentrifugasi Menggunakan Diluter Skim Kuning Telur Dan Lesitin Kacang Kedelai
title_sort measurement of nuclear dna damage of simental bull spermatozoa post thawing with sentrifuged egg yolk skim and soybean lecitin diluter = pengukuran kerusakan dna inti spermatozoa sapi simental post thawing yang disentrifugasi menggunakan diluter skim kuning telur dan lesitin kacang kedelai
publisher Departemen Reproduksi Fakultas Kedokteran Hewan
publishDate 2014
url http://repository.unair.ac.id/59267/1/karil%2024%20%28pengukuran%20kerusakan%20dna%20inti%29.pdf
http://repository.unair.ac.id/59267/2/peerreview%20karil%2024%20%28pengukuran%20kerusakan%29.pdf
http://repository.unair.ac.id/59267/
http://journal.unair.ac.id/measurement-of-nuclear-dna-damage-of-simental-bull-spermatozoa-post-thawing-with-sentrifuged-egg-yolk-skim-and-soybean-lecitin-diluter-article-7966-media-123-category-5.html
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