ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT

The purpose of this research to get the specific protein from adult stage of Toxocara vitulorum used Whole Worm Extract (WWE) as antigen which can be used for toxocariasis diagnostic serologically. Toxocara vitulorum was collected from gut cattle. To homogenize WWE, mortar and stamfer are used, t...

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Main Author: DESY META ANGGRAINI, 061311133025
Format: Theses and Dissertations NonPeerReviewed
Language:English
Indonesian
Published: 2017
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Online Access:http://repository.unair.ac.id/60934/1/ABSTRACT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf
http://repository.unair.ac.id/60934/2/FULLTEXT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf
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Institution: Universitas Airlangga
Language: English
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spelling id-langga.609342017-08-24T23:05:15Z http://repository.unair.ac.id/60934/ ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT DESY META ANGGRAINI, 061311133025 QL1-991 Zoology QP501-801 Animal biochemistry The purpose of this research to get the specific protein from adult stage of Toxocara vitulorum used Whole Worm Extract (WWE) as antigen which can be used for toxocariasis diagnostic serologically. Toxocara vitulorum was collected from gut cattle. To homogenize WWE, mortar and stamfer are used, then added by 2 ml of PBS, next WWE homogenized by centrifuge 4.000 rpm while 15 minutes. Initiation and booster were done to get policlonal antibody from mice. Indirect ELISA used to measure sera antibody levels. Separation of protein by discontinuous system of SDS-PAGE technique (12% separating gel and 5% stacking gel). Characterization of specific protein by semi dry of Western blot technique with western blue staining used sera anti-Mecistocirrus digitatus and IgG anti-mouse. The result of this research are the protein’s bands which each of them have molecular weight. The result showed the existence of cross-reaction between WWE T. vitulorum antigen with anti-M. digitatus serum in a protein with a molecular weight of 176, 124, 92, 68, 59, 47, 31, 29, 26, 16, 12 and 10 kDa. 2017 Thesis NonPeerReviewed text en http://repository.unair.ac.id/60934/1/ABSTRACT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf text id http://repository.unair.ac.id/60934/2/FULLTEXT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf DESY META ANGGRAINI, 061311133025 (2017) ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT. Skripsi thesis, Universitas Airlangga. http://lib.unair.ac.id
institution Universitas Airlangga
building Universitas Airlangga Library
country Indonesia
collection UNAIR Repository
language English
Indonesian
topic QL1-991 Zoology
QP501-801 Animal biochemistry
spellingShingle QL1-991 Zoology
QP501-801 Animal biochemistry
DESY META ANGGRAINI, 061311133025
ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT
description The purpose of this research to get the specific protein from adult stage of Toxocara vitulorum used Whole Worm Extract (WWE) as antigen which can be used for toxocariasis diagnostic serologically. Toxocara vitulorum was collected from gut cattle. To homogenize WWE, mortar and stamfer are used, then added by 2 ml of PBS, next WWE homogenized by centrifuge 4.000 rpm while 15 minutes. Initiation and booster were done to get policlonal antibody from mice. Indirect ELISA used to measure sera antibody levels. Separation of protein by discontinuous system of SDS-PAGE technique (12% separating gel and 5% stacking gel). Characterization of specific protein by semi dry of Western blot technique with western blue staining used sera anti-Mecistocirrus digitatus and IgG anti-mouse. The result of this research are the protein’s bands which each of them have molecular weight. The result showed the existence of cross-reaction between WWE T. vitulorum antigen with anti-M. digitatus serum in a protein with a molecular weight of 176, 124, 92, 68, 59, 47, 31, 29, 26, 16, 12 and 10 kDa.
format Theses and Dissertations
NonPeerReviewed
author DESY META ANGGRAINI, 061311133025
author_facet DESY META ANGGRAINI, 061311133025
author_sort DESY META ANGGRAINI, 061311133025
title ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT
title_short ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT
title_full ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT
title_fullStr ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT
title_full_unstemmed ANTIGENITAS PROTEIN Toxocara vitulorum DENGAN SERUM ANTI-Mecistocirrus digitatus MENGGUNAKAN TEKNIK WESTERN BLOT
title_sort antigenitas protein toxocara vitulorum dengan serum anti-mecistocirrus digitatus menggunakan teknik western blot
publishDate 2017
url http://repository.unair.ac.id/60934/1/ABSTRACT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf
http://repository.unair.ac.id/60934/2/FULLTEXT%20KKC%20KK%20KH.%20172.17%20Ang%20a.pdf
http://repository.unair.ac.id/60934/
http://lib.unair.ac.id
_version_ 1681148207172681728