Lignocellulosic Enzymes Characterization and Scanning Electron Microscope Analysis on Rice Bran Surface Structure Changes
Rice bran was one of livestock food ingredients wit h high crude fibers contents, including cellulose and hemicelluloses. Lignocellulosic enzymes such as xylanase and cellulase functioned in degrading cellulose and hemicelluloses. The objectives of this study were to qu...
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Main Authors: | , , , , , |
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Format: | Article PeerReviewed |
Language: | English English |
Published: |
www.jocpr.com
2015
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Online Access: | http://repository.unair.ac.id/63554/3/Peereview%20018.pdf http://repository.unair.ac.id/63554/2/lignocellulosic-enzymes-characterization-and-scanning-electron-microscope-analysis-on-rice-bran-surface-structure-change.pdf http://repository.unair.ac.id/63554/ http://www.jocpr.com/articles/lignocellulosic-enzymes-characterization-and-scanning-electron-microscope-analysis-on-rice-bran-surface-structure-change.pdf |
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Institution: | Universitas Airlangga |
Language: | English English |
Summary: | Rice bran was one of livestock food ingredients wit
h high crude fibers contents, including cellulose and
hemicelluloses. Lignocellulosic enzymes such as xylanase and cellulase functioned in degrading cellulose and hemicelluloses. The objectives of this study were to qualitatively screen Actinobacillus sp. (G6) and
Bacillus pumilus (G7) which produced xylanase and cellulase, determining molecular weight of xylanase and cellulase enz
ymes using SDS-PAGE and Zymogram, and analyzing structure changes on rice bran surface after Scanning Electron Microscope treatments using xylanase and cellulase
enzymes. The findings of this study indicated xylanolitic indexes for G6 and G7 were 2 and 1.7 respectively while cel
lulosic index for G6 and G7 were 1.6 and 2. Enzymesproduced
by Actinobacillus sp. (G6) had molecular weight 25-
35 kDa and above 100 kDa while enzymes produced by
Bacillus pumilus (G7) had molecular weight 25 kDa up to above 100 kDa. The result of rice bran SEM analysis indicated profile change of the samples before and after enzymes treatment |
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