AKTIVITAS ANTIMALARIA FRAKSI ETIL ASETAT-96 DAN FRAKSI ETIL ASETAT-70 HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NEES) SECARA IN VIVO PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI
The issue of resistance in malarial infection makes development of novel drugs a necessity. An alternative source for discovering such drugs is natural products. Andrographis paniculata Nees which known as sambiloto was traditionally used for antimalaria in Indonesia. Therefore, the aim for this...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/65088/1/FF.FT.03.17%20.%20Ast.a%20-%20ABSTRAK.pdf http://repository.unair.ac.id/65088/2/FF.FT.03.17%20.%20Ast.a%20-%20SEC.pdf http://repository.unair.ac.id/65088/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian |
| Summary: | The issue of resistance in malarial infection makes
development of novel drugs a necessity. An alternative source for
discovering such drugs is natural products. Andrographis paniculata
Nees which known as sambiloto was traditionally used for
antimalaria in Indonesia. Therefore, the aim for this study was to
develop A. paniculata as a new phytopharmaceutical drug. The study
was began for determining antimalaria acitivity of raw material
product. The material are Etil Asetat fraction that obtained from
ethanol extract 96% and ethanol extract 70% of A. paniculata herbs.
Then comparing between the two materials that give better activity
determined by the value of each ED50 as reference to be
manufactured as phytopharmaceutical drug. In vivo antimalarial
assay on Plasmodium berghei infected mice was carried out by oral
administration, twice a day for four consecutive days based on
Peter’s 4 days suppressive test. Each EA fraction was given at a dose
which equal to 6.25; 12.5; 25; and 50 mg andrographolide per kg
mice body weight. The observation was done for a week (H0-H6) and
everyday thin films were made from the tail blood of each mouse. The parasitemia level was determined by counting the number of
parasitized erythrocytes out of 1000 normal erythrocytes in random
fields of the microscope. Antimalarial test results showed that
antimalarial activity of EA fraction-96 higher than EA fraction-70
with ED50 4.216 mg/kg BW and 6.747 mg/kg BW respectively. |
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